Transcriptomic analysis reveals the role of FOUR LIPS in response to salt stress in rice

doi:10.1007/s11103-022-01282-9

	Transcriptomic analysis reveals the role of FOUR LIPS in response to salt stress in rice
	Zhang, Chunxia ; Zhang, Jie 1; Liu, Huichao 1; Qu, Xiaoxiao 1; Wang, Junxue 1,3; He, Qixiumei 1; Zou, Junjie 2; Yang, Kezhen ; Le, Jie1
	2022
发表期刊	PLANT MOLECULAR BIOLOGY
ISSN	0167-4412
卷号	110 期号:1-2 页码:37-52
摘要	Key message An R2R3-MYB transcription factor FOUR LIPS associated with B-type Cyclin-Dependent Kinase 1;1 confers salt tolerance in rice. The Arabidopsis FOUR LIPS (AtFLP), an R2R3 MYB transcription factor, acts as an important stomatal development regulator. Only one orthologue protein of AtFLP, Oryza sativa FLP (OsFLP), was identified in rice. However, the function of OsFLP is largely unknown. In this study, we conducted RNA-seq and ChIP-seq to investigate the potential role of OsFLP in rice. Our results reveal that OsFLP is probably a multiple functional regulator involved in many biological processes in growth development and stress responses in rice. However, we mainly focus on the role of OsFLP in salt stress response. Consistently, phenotypic analysis under salt stress conditions showed that osflp exhibited significant sensitivity to salt stress, while OsFLP over-expression lines displayed obvious salt tolerance. Additionally, Yeast one-hybrid assay and electrophoretic mobility shift assay (EMSA) showed that OsFLP directly bound to the promoter region of Oryza sativa B-type Cyclin-Dependent Kinase 1;1 (OsCDKB1;1), and the expression of OsCDKB1;1 was repressed in osflp. Disturbing the expression of OsCDKB1;1 remarkably enhanced the tolerance to salt stress. Taken together, our findings reveal a crucial function of OsFLP regulating OsCDKB1;1 in salt tolerance and largely extend the knowledge about the role of OsFLP in rice.
关键词	RNA-seq ChIP-seq OsFLP OsCDKB1 1 Rice Salt stress
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1007/s11103-022-01282-9
收录类别	SCI
语种	英语
WOS关键词	CYCLIN-DEPENDENT-KINASES ; MEDIATED REGULATION ; OXIDATIVE STRESS ; ION HOMEOSTASIS ; ARABIDOPSIS ; TOLERANCE ; SALINITY ; DROUGHT ; GENES ; COLD
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000797274600001
出版者	SPRINGER
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5028
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences [XDA24010303] ; National Natural Science Foundation of China [31970804, 32170722, 31470362, 31871377, 32070723]
作者邮箱	spring1984@163.com ; jieer1027@163.com ; liuhuichao@ibcas.ac.cn ; quxiaoxiao721@sina.com ; wangjunxues@163.com ; heqixiumei@ibcas.ac.cn ; zoujunjie@caas.cn ; ykzdsp@163.com ; lejie@ibcas.ac.cn
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28603
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China 4.Wenbo Sch, Jinan 250100, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Chunxia,Zhang, Jie,Liu, Huichao,et al. Transcriptomic analysis reveals the role of FOUR LIPS in response to salt stress in rice[J]. PLANT MOLECULAR BIOLOGY,2022,110(1-2):37-52.
APA	Zhang, Chunxia.,Zhang, Jie.,Liu, Huichao.,Qu, Xiaoxiao.,Wang, Junxue.,...&Le, Jie.(2022).Transcriptomic analysis reveals the role of FOUR LIPS in response to salt stress in rice.PLANT MOLECULAR BIOLOGY,110(1-2),37-52.
MLA	Zhang, Chunxia,et al."Transcriptomic analysis reveals the role of FOUR LIPS in response to salt stress in rice".PLANT MOLECULAR BIOLOGY 110.1-2(2022):37-52.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Zhang-2022-Transcrip（3261KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文