Scaling-up and proteomic analysis reveals photosynthetic and metabolic insights toward prolonged H2 photoproduction in Chlamydomonas hpm91 mutant lacking proton gradient regulation 5 (PGR5)

doi:10.1007/s11120-022-00945-4

IB-CAS > 中科院光生物学重点实验室

	Scaling-up and proteomic analysis reveals photosynthetic and metabolic insights toward prolonged H2 photoproduction in Chlamydomonas hpm91 mutant lacking proton gradient regulation 5 (PGR5)
	Liu, Peng ; Ye, De-Min ; Chen, Mei ; Zhang, Jin ; Huang, Xia-He ; Shen, Li-Li ; Xia, Ke-Ke ; Xu, Xiao-Jing ; Xu, Yong-Chao ; Guo, Ya-Long; Wang, Ying-Chun ; Huang, Fang5
	2022
发表期刊	PHOTOSYNTHESIS RESEARCH
ISSN	0166-8595
卷号	154 期号:3 页码:397-411
摘要	Clean and sustainable H-2 production is crucial to a carbon-neutral world. H-2 generation by Chlamydomonas reinhardtii is an attractive approach for solar-H-2 from H2O. However, it is currently not large-scalable because of lacking desirable strains with both optimal H-2 productivity and sufficient knowledge of underlying molecular mechanism. We hereby carried out extensive and in-depth investigations of H-2 photoproduction of hpm91 mutant lacking PGR5 (Proton Gradient Regulation 5) toward its up-scaling and fundamental mechanism issues. We show that hpm91 is at least 100-fold scalable (up to 10 L) with continuous H-2 collection of 7287 ml H-2/10L-HPBR in averagely 26 days under sulfur deprivation. Also, we show that hpm91 is robust and active during sustained H-2 photoproduction, most likely due to decreased intracellular ROS relative to wild type. Moreover, we obtained quantitative proteomic profiles of wild type and hpm91 at four representing time points of H-2 evolution, leading to 2229 and 1350 differentially expressed proteins, respectively. Compared to wild type, major proteome alterations of hpm91 include not only core subunits of photosystems and those related to anti-oxidative responses but also essential proteins in photosynthetic antenna, C/N metabolic balance, and sulfur assimilation toward both cysteine biosynthesis and sulfation of metabolites during sulfur-deprived H-2 production. These results reveal not only new insights of cellular and molecular basis of enhanced H-2 production in hpm91 but also provide additional candidate gene targets and modules for further genetic modifications and/or in artificial photosynthesis mimics toward basic and applied research aiming at advancing solar-H-2 technology.
关键词	C. reinhardtii hpm91 mutant Scale-up H-2 production ROS Quantitative proteomics hpm91-derived mutant
学科领域	Plant Sciences
DOI	10.1007/s11120-022-00945-4
收录类别	SCI
语种	英语
WOS关键词	CYCLIC ELECTRON FLOW ; HYDROGEN-PRODUCTION ; GREEN-ALGA ; PHOTOSYSTEM-II ; NITROGEN-METABOLISM ; REINHARDTII ; LIGHT ; PATHWAYS ; PROTEIN ; ENERGY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000841042100001
出版者	SPRINGER
Special Issue	SI
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5079
资助机构	National Natural Science Foundation of China [31470340] ; Ministry of Science and Technology of China [2015CB150100] ; CAS [XDB17030300, KGCX2-YW-373]
作者邮箱	ycwang@genetics.ac.cn ; fhuang@ibcas.ac.cn
作品OA属性	hybrid, Green Published
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28609
专题	中科院光生物学重点实验室
作者单位	1.[Liu, Peng 2.Ye, De-Min 3.Chen, Mei 4.Zhang, Jin 5.Shen, Li-Li 6.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 7.[Huang, Xia-He 8.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China 9.[Xia, Ke-Ke 10.BGI Shenzhen, Shenzhen 518083, Peoples R China 11.[Xu, Yong-Chao 12.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 13.Xu, Xiao-Jing 14.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Peng,Ye, De-Min,Chen, Mei,et al. Scaling-up and proteomic analysis reveals photosynthetic and metabolic insights toward prolonged H2 photoproduction in Chlamydomonas hpm91 mutant lacking proton gradient regulation 5 (PGR5)[J]. PHOTOSYNTHESIS RESEARCH,2022,154(3):397-411.
APA	Liu, Peng.,Ye, De-Min.,Chen, Mei.,Zhang, Jin.,Huang, Xia-He.,...&Huang, Fang.(2022).Scaling-up and proteomic analysis reveals photosynthetic and metabolic insights toward prolonged H2 photoproduction in Chlamydomonas hpm91 mutant lacking proton gradient regulation 5 (PGR5).PHOTOSYNTHESIS RESEARCH,154(3),397-411.
MLA	Liu, Peng,et al."Scaling-up and proteomic analysis reveals photosynthetic and metabolic insights toward prolonged H2 photoproduction in Chlamydomonas hpm91 mutant lacking proton gradient regulation 5 (PGR5)".PHOTOSYNTHESIS RESEARCH 154.3(2022):397-411.

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