The transcription factor OsGATA6 regulates rice heading date and grain number per panicle

doi:10.1093/jxb/erac247

	The transcription factor OsGATA6 regulates rice heading date and grain number per panicle
	Zhang, Yan-Jie ; Zhang, Yu ; Zhang, Liang-Li ; He, Jun-Xian ; Xue, Hong-Wei ; Wang, Jia-Wei ; Lin, Wen-Hui
	2022
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	73 期号:18 页码:6133-6149
摘要	Heading date, panicle architecture, and grain size are key traits that affect the yield of rice (Oryza sativa). Here, we identified a new gene, OsGATA6, whose product regulates heading date. Overexpression of OsGATA6 resulted in delayed heading, increased grain number, and decreased grain size. Knockdown lines generated by artificial microRNA (amiRNA) and CRISPR genome-edited lines of OsGATA6 both showed earlier heading, decreased grain number, and increased grain size. These results suggested that OsGATA6 negatively regulates heading date, positively regulates panicle development, and affects grain size. OsGATA6 was found to be constitutively expressed in rice, and strongly expressed in young leaves and panicles. In situ hybridization analyses showed that OsGATA6 was specifically localized in superficial cells of the panicle primordium. Overexpression lines show decreased expression of RFT1 and Hd3a, which promote heading. OsMFT1, which delays heading date and increases grain number, was down-regulated in amiRNA lines. Further analyses showed that OsGATA6 could bind to the promoter of OsMFT1 and induce its expression, thereby regulating heading date and panicle development. Overexpression of OsGATA6 in Arabidopsis resulted in repressed expression of AtFT and late flowering, suggesting that its function is similar. Taken together, we have identified a new GATA regulator that influences rice heading date and grain number, which potentially increases rice yield. OsGATA6inhibits rice heading and increases grain number through transcriptional regulation of MFT1, and its overexpression has the potential to enhance yield through increasing grain number.
关键词	Florigen GATA transcription factor grain number per panicle grain size heading date Oryza sativa rice
学科领域	Plant Sciences
DOI	10.1093/jxb/erac247
收录类别	SCI
语种	英语
WOS关键词	PHOTOPERIODIC CONTROL ; SIGNALING PATHWAYS ; NATURAL VARIATION ; ROOT DEVELOPMENT ; GATA FAMILY ; SEED SIZE ; GENES ; YIELD ; ARCHITECTURE ; PROTEIN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000826577900001
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	National Natural Science Foundation of China (NSFC) [31761163003, 90817009, 32000222] ; Ministry of Agriculture of China [2009ZX08009-022B, 2008ZX08009-003] ; National Basic Research Program of China [2014CB943404] ; Agri-X Interdisciplinary Fund of Shanghai Jiao Tong University [Agri-X20200204] ; Shanghai Jiao Tong University JiRLMDS Joint Research Fund [MDS-JF-2020-8] ; NSFC-Research Grants Council of Hong Kong (RGC) Joint Scheme [NCUHK452/17]
作者邮箱	whlin@sjtu.edu.cn
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28641
专题	中科院植物分子生理学重点实验室
作者单位	1.[Zhang, Yan-Jie 2.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Joint Int Res Lab Metab & Dev Sci, Shanghai 200240, Peoples R China 3.Xue, Hong-Wei 4.Shanghai Jiao Tong Univ, Shanghai Collaborat Innovat Ctr AgriSeeds, Joint Ctr Single Cell Biol, Shanghai 200240, Peoples R China 5.[Zhang, Yu 6.Chinese Acad Sci, Inst Bot, State Key Lab Plant Mol Physiol, Beijing, Peoples R China 7.Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong, Peoples R China 8.Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Shatin, Hong Kong, Peoples R China 9.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China 10.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci CEMPS, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yan-Jie,Zhang, Yu,Zhang, Liang-Li,et al. The transcription factor OsGATA6 regulates rice heading date and grain number per panicle[J]. JOURNAL OF EXPERIMENTAL BOTANY,2022,73(18):6133-6149.
APA	Zhang, Yan-Jie.,Zhang, Yu.,Zhang, Liang-Li.,He, Jun-Xian.,Xue, Hong-Wei.,...&Lin, Wen-Hui.(2022).The transcription factor OsGATA6 regulates rice heading date and grain number per panicle.JOURNAL OF EXPERIMENTAL BOTANY,73(18),6133-6149.
MLA	Zhang, Yan-Jie,et al."The transcription factor OsGATA6 regulates rice heading date and grain number per panicle".JOURNAL OF EXPERIMENTAL BOTANY 73.18(2022):6133-6149.

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