AtNusG, a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana

doi:10.1093/nar/gkac501

IB-CAS > 中科院光生物学重点实验室

	AtNusG, a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana
	Xiong, Hai-Bo ; Pan, Hui-Min ; Long, Qiao-Ying ; Wang, Zi-Yuan ; Qu, Wan-Tong ; Mei, Tong ; Zhang, Nan ; Xu, Xiao-Feng ; Yang, Zhong-Nan ; Yu, Qing-Bo
	2022
发表期刊	NUCLEIC ACIDS RESEARCH
ISSN	0305-1048
卷号	50 期号:12 页码:6715-6734
摘要	In Escherichia coli, transcription-translation coupling is mediated by NusG. Although chloroplasts are descendants of endosymbiotic prokaryotes, the mechanism underlying this coupling in chloroplasts remains unclear. Here, we report transcription-translation coupling through AtNusG in chloroplasts. AtNusG is localized in chloroplast nucleoids and is closely associated with the chloroplast PEP complex by interacting with its essential component PAP9. It also comigrates with chloroplast ribosomes and interacts with their two components PRPS5 (uS5c) and PRPS10 (uS10c). These data suggest that the transcription and translation machineries are coupled in chloroplasts. In the atnusg mutant, the accumulation of chloroplast-encoded photosynthetic gene transcripts, such as psbA, psbB, psbC and psbD, was not obviously changed, but that of their proteins was clearly decreased. Chloroplast polysomic analysis indicated that the decrease in these proteins was due to the reduced efficiency of their translation in this mutant, leading to reduced photosynthetic efficiency and enhanced sensitivity to cold stress. These data indicate that AtNusG-mediated coupling between transcription and translation in chloroplasts ensures the rapid establishment of photosynthetic capacity for plant growth and the response to environmental changes. Therefore, our study reveals a conserved mechanism of transcription-translation coupling between chloroplasts and E. coli, which perhaps represents a regulatory mechanism of chloroplast gene expression. This study provides insights into the underlying mechanisms of chloroplast gene expression in higher plants.
学科领域	Biochemistry & Molecular Biology
DOI	10.1093/nar/gkac501
收录类别	SCI
语种	英语
WOS关键词	ACTIVE CHROMOSOME COMPLEX ; RIBOSOMAL-PROTEIN ; FACTOR-NUSG ; INTERACTS ; STRESS ; VISUALIZATION ; COMPONENT ; PLASTIDS ; SEQUENCE ; BINDING
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000814749800001
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1362-4962
资助机构	National Natural Scientific Foundation of China [31570232, 31930009] ; Shanghai Key Laboratory of Plant Molecular Sciences
作者邮箱	znyang@shnu.edu.cn ; yuqing9860@shnu.edu.cn
作品OA属性	Green Published, gold
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28647
专题	中科院光生物学重点实验室
作者单位	1.[Xiong, Hai-Bo 2.Pan, Hui-Min 3.Long, Qiao-Ying 4.Wang, Zi-Yuan 5.Qu, Wan-Tong 6.Mei, Tong 7.Zhang, Nan 8.Xu, Xiao-Feng 9.Yang, Zhong-Nan 10.Shanghai Normal Univ, Coll Life Sci, Shanghai Key Lab Plant Mol Sci, Shanghai 200234, Peoples R China 11.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 12.Henan Univ, Sch Life Sci, Kaifeng 475004, Henan, Peoples R China
推荐引用方式 GB/T 7714	Xiong, Hai-Bo,Pan, Hui-Min,Long, Qiao-Ying,et al. AtNusG, a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana[J]. NUCLEIC ACIDS RESEARCH,2022,50(12):6715-6734.
APA	Xiong, Hai-Bo.,Pan, Hui-Min.,Long, Qiao-Ying.,Wang, Zi-Yuan.,Qu, Wan-Tong.,...&Yu, Qing-Bo.(2022).AtNusG, a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana.NUCLEIC ACIDS RESEARCH,50(12),6715-6734.
MLA	Xiong, Hai-Bo,et al."AtNusG, a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana".NUCLEIC ACIDS RESEARCH 50.12(2022):6715-6734.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Xiong-2022-AtNusG, a（6246KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文