Waxy is an important factor for grain fissure resistance and head rice yield as revealed by a genome-wide association study

doi:10.1093/jxb/erac330

	Waxy is an important factor for grain fissure resistance and head rice yield as revealed by a genome-wide association study
	Deng, Zhuyun ; Liu, Yuxia 1; Gong, Chunyan ; Chen, Bingtang ; Wang, Tai1,2
	2022
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	73 期号:19 页码:6942-6954
摘要	The amylose synthase gene Waxyaffects grain fissure resistance and head rice yield by altering the layered structure of starch granules, thereby affecting their swelling response to moisture. Head rice yield (HRY) is an essential quality trait, and is sensitive to environmental stresses during the grain-filling, harvest, and postharvest stages. It is therefore important for rice production and global food security to select for superior HRY traits; however, the molecular basis of this trait remains unknown. Using diverse rice germplasm material, we performed a genome-wide association study of grain fissure resistance (GFR), the phenotype most associated with HRY, and found that the granule-bound starch synthase I gene Waxy is an important gene controlling GFR. Analysis of near-isogenic lines demonstrated that genetic variations in Waxy conferred different levels of tolerance to fissuring in grains. The null allele wx resulted in the highest GFR, while alleles that increased amylose synthesis reduced GFR. Increases in amylose content led to increases in the ratio of the widths of the amorphous layer to the semi-crystalline layer of the starch granules, and also to increased occurrence of chalkiness. The layer structure determined GFR by affecting the degree of swelling of granules in response to moisture, and chalkiness acted as an accelerator of moisture infiltration to rapidly increase the number of swelling granules. Our study reveals the molecular basis of GFR and HRY, thus opening the door for further understanding of the molecular networks of GFR and HRY.
关键词	Amylose genetic variations genome-wide association study grain fissure resistance head rice yield near-isogenic lines Oryza sativa rice germplasm starch granules Waxy
学科领域	Plant Sciences
DOI	10.1093/jxb/erac330
收录类别	SCI
语种	英语
WOS关键词	QUANTITATIVE TRAIT LOCI ; KERNEL ; QUALITY ; STATE ; GENE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000848814300001
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	Chinese Academy of Sciences [XDA24010103] ; Ministry of Science and Technology of the People's Republic of China [2020YFE0202300, 2016YFD0100604]
作者邮箱	twang@ibcas.ac.cn
作品OA属性	Green Published
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28651
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Deng, Zhuyun,Liu, Yuxia,Gong, Chunyan,et al. Waxy is an important factor for grain fissure resistance and head rice yield as revealed by a genome-wide association study[J]. JOURNAL OF EXPERIMENTAL BOTANY,2022,73(19):6942-6954.
APA	Deng, Zhuyun,Liu, Yuxia,Gong, Chunyan,Chen, Bingtang,&Wang, Tai.(2022).Waxy is an important factor for grain fissure resistance and head rice yield as revealed by a genome-wide association study.JOURNAL OF EXPERIMENTAL BOTANY,73(19),6942-6954.
MLA	Deng, Zhuyun,et al."Waxy is an important factor for grain fissure resistance and head rice yield as revealed by a genome-wide association study".JOURNAL OF EXPERIMENTAL BOTANY 73.19(2022):6942-6954.

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