MICROMORPHOLOGY, PHYTOCHEMICAL AND PHARMACOLOGICAL EVALUATION OF Isodon rugosus (Wail. ex Benth.) Codd

doi:10.36899/JAPS.2022.3.0475

	MICROMORPHOLOGY, PHYTOCHEMICAL AND PHARMACOLOGICAL EVALUATION OF Isodon rugosus (Wail. ex Benth.) Codd
	Hameed, A.; Zafar, M.; Ahmad, M.; Sultana, S.; Akhter, M. S.; Zaman, W.; Saqib, S.; Ullah, F.
	2022
发表期刊	JOURNAL OF ANIMAL AND PLANT SCIENCES-JAPS
ISSN	1018-7081
卷号	32 期号:3 页码:736-745
摘要	Isodon rugosus Wall. ex Benth (IR) is one of the ethnomedicinal important plants of Gilgit-Baltistan, Northern Pakistan. The present study aims to evaluate the micro-morphological features, phytochemical screening, and pharmacological potential of IR. SEM and LM were used as an identification tool. Five different solvents were used to prepare IR extracts. Phytochemical and antioxidant activities were determined calorimetrically. To investigate antidiabetic, alpha-amylase inhibition assay was adopted. Cytotoxicity was tested using a brine shrimp assay. Anti-leishmanial via MTT assay. Disc-diffusion assay was used for protein kinase inhibitory, antibacterial, and antifungal activities. Pollen was of monad, hexacolporate, and circular shape. Lophate sculpturing with outer exine pattern elevated on the rides. Seeds were oblong-ovate, small projected with smooth-rough, variously ridges/wrinkled sculpturing. Epidermis cells were irregular in shape with a slightly straight anticlinal wall. Stomata with diacytic, and unicellular glandular trichome were observed. The extracts were rich in phytochemicals, the maximum amount of phenolic and flavonoid contents was found in methanol extract (IRM) 89.76mg GAE/gm and 85.69mg QE/gm. All the extracts show substantial antioxidant activity but highest in IRM (DPPH IC50 44.51 mu g/ml, total antioxidant capacity 93.60 mg AAE/g, and total reduction power of 93.44 mg AAE/g). Potential antibacterial and antifungal activities were reported for IRM. Significant protein kinase, alpha-amylase inhibition, and cytotoxic activity were revealed. Dose-dependent cytotoxic activity was exposed against Leishmania tropica (LC50 11.16 mu g/mL). In conclusion, I. rugosus extracts have shown potential biological applications and should be subjected to further research work to develop further biomedical applications.
关键词	Isodon rugosus SEM LM phytochemicals antimicrobial cytotoxicity alpha-amylase inhibition
学科领域	Agriculture, Multidisciplinary ; Biology ; Veterinary Sciences
DOI	10.36899/JAPS.2022.3.0475
收录类别	SCI
语种	英语
WOS关键词	ANTIOXIDANT ; EXTRACT ; IDENTIFICATION ; NANOPARTICLES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000806392400014
出版者	PAKISTAN AGRICULTURAL SCIENTISTS FORUM
文献子类	Article
出版地	LAHORE
EISSN	2309-8694
作者邮箱	fazalbotanist@gmail.com
作品OA属性	hybrid
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28656
专题	系统与进化植物学国家重点实验室
作者单位	1.[Hameed, A. 2.Zafar, M. 3.Ahmad, M. 4.Sultana, S.] Quaid I Azam Univ, Dept Plant Sci, Islamabad, Pakistan 5.[Akhter, M. S.] Univ Bahrain, Coll Sci, Dept Chem, Zallaq, Bahrain 6.[Zaman, W.] Yeungnam Univ, Dept Life Sci, Gyongsan 38541, Gyeongbuk, South Korea 7.[Saqib, S.] Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 8.[Saqib, S. 9.Ullah, F.] Univ Chinese Acad Sci, Beijing 100049, Peoples R China 10.[Ullah, F.] Chinese Acad Sci, Chengdu Inst Biol, CAS Key Lab Mt Ecol Restorat & Bioresource Utiliz, Chengdu, Peoples R China
推荐引用方式 GB/T 7714	Hameed, A.,Zafar, M.,Ahmad, M.,et al. MICROMORPHOLOGY, PHYTOCHEMICAL AND PHARMACOLOGICAL EVALUATION OF Isodon rugosus (Wail. ex Benth.) Codd[J]. JOURNAL OF ANIMAL AND PLANT SCIENCES-JAPS,2022,32(3):736-745.
APA	Hameed, A..,Zafar, M..,Ahmad, M..,Sultana, S..,Akhter, M. S..,...&Ullah, F..(2022).MICROMORPHOLOGY, PHYTOCHEMICAL AND PHARMACOLOGICAL EVALUATION OF Isodon rugosus (Wail. ex Benth.) Codd.JOURNAL OF ANIMAL AND PLANT SCIENCES-JAPS,32(3),736-745.
MLA	Hameed, A.,et al."MICROMORPHOLOGY, PHYTOCHEMICAL AND PHARMACOLOGICAL EVALUATION OF Isodon rugosus (Wail. ex Benth.) Codd".JOURNAL OF ANIMAL AND PLANT SCIENCES-JAPS 32.3(2022):736-745.

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