Knowledge Management System Of Institute Of Botany,CAS
| OsMADS14 and NF-YB1 cooperate in the direct activation of OsAGPL2 and Waxy during starch synthesis in rice endosperm | |
| Feng, Tingting1; Wang, Lili1; Li, Laiyun1; Liu, Yuan; Chong, Kang; Theissen, Guenter2; Meng, Zheng | |
| 2022 | |
| 发表期刊 | NEW PHYTOLOGIST
 |
| ISSN | 0028-646X |
| 卷号 | 234期号:1页码:77-92 |
| 摘要 | Starch synthesis makes a dramatic contribution to the yield and nutritional value of cereal crops. Although several starch synthesis enzymes and related regulators have been reported, the underlying regulatory mechanisms of starch synthesis remain largely unknown. OsMADS14 is a FRUITFULL (FUL)-like MADS-box gene in rice (Oryza sativa). Here we show that two null mutations of OsMADS14 result in a shrunken and chalky grain phenotype. It is caused by obviously defective compound starch granules and a significantly reduced content of both total starch and amylose in the endosperm. Transcriptomic profiling analyses revealed that the loss-of-function of OsMADS14 leads to significantly downregulated expression of many core starch synthesis genes, including OsAGPL2 and Waxy. Both in vitro and in vivo assays demonstrate that the OsMADS14 protein directly binds to stretches of DNA with a CArG-box consensus in the putative regulatory regions of OsAGPL2 and Waxy. Protein-protein interaction experiments also suggest that OsMADS14 interacts with nuclear factor NF-YB1 to promote the transcription of OsAGPL2 and Waxy. Our study thus demonstrates that OsMADS14 plays an essential role in the synthesis of storage starch and provides novel insights into the underlying molecular mechanism that may be used to improve rice cultivars by molecular breeding. |
| 关键词 | endosperm MADS-box gene NF-YB1 Oryza sativa OsMADS14 protein-protein interaction starch synthesis |
| 学科领域 | Plant Sciences |
| DOI | 10.1111/nph.17990 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | ADP-GLUCOSE PYROPHOSPHORYLASE ; MADS-BOX GENES ; TRANSCRIPTION FACTORS ; MERISTEM IDENTITY ; AMYLOSE CONTENT ; PROTEIN ; BIOSYNTHESIS ; ARABIDOPSIS ; EXPRESSION ; FRUITFULL |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000754301500001 |
| 出版者 | WILEY |
| 文献子类 | Article |
| 出版地 | HOBOKEN |
| EISSN | 1469-8137 |
| 资助机构 | National Natural Science Foundation of China [31771364] |
| 作者邮箱 | guenter.theissen@uni-jena.de ; zhmeng@ibcas.ac.cn |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/28665 |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Friedrich Schiller Univ Jena, Matthias Schleiden Inst, Genet, D-07743 Jena, Germany |
| 推荐引用方式 GB/T 7714 | Feng, Tingting,Wang, Lili,Li, Laiyun,et al. OsMADS14 and NF-YB1 cooperate in the direct activation of OsAGPL2 and Waxy during starch synthesis in rice endosperm[J]. NEW PHYTOLOGIST,2022,234(1):77-92. |
| APA | Feng, Tingting.,Wang, Lili.,Li, Laiyun.,Liu, Yuan.,Chong, Kang.,...&Meng, Zheng.(2022).OsMADS14 and NF-YB1 cooperate in the direct activation of OsAGPL2 and Waxy during starch synthesis in rice endosperm.NEW PHYTOLOGIST,234(1),77-92. |
| MLA | Feng, Tingting,et al."OsMADS14 and NF-YB1 cooperate in the direct activation of OsAGPL2 and Waxy during starch synthesis in rice endosperm".NEW PHYTOLOGIST 234.1(2022):77-92. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| OsMADS14_and_NF_YB1_（3111KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论