OsGLYI3, a glyoxalase gene expressed in rice seed, contributes to seed longevity and salt stress tolerance

doi:10.1016/j.plaphy.2022.04.028

	OsGLYI3, a glyoxalase gene expressed in rice seed, contributes to seed longevity and salt stress tolerance
	Liu, Shengjie 1; Liu, Wenhua 1; Lai, Jianyun 1; Liu, Qinjian 1; Zhang, Wenhu 1; Chen, Zhongjian 1; Gao, Jiadong 1; Song, Songquan 2; Liu, Jun 1; Xiao, Yinghui
	2022
发表期刊	PLANT PHYSIOLOGY AND BIOCHEMISTRY
ISSN	0981-9428
卷号	183 页码:85-95
摘要	The glyoxalase pathway plays a vital role in the chemical detoxification of methylglyoxal (MG) in biological systems. Our previous study suggested that OsGLYI3 may be effective in seed natural aging. In this study, the rice OsGLYI3 gene was cloned and characterized as specifically expressed in the seed. The accelerated aging (AA) treatment results indicated significant roles of OsGLYI3 in seed longevity and vigor, as the seeds of the transgenic lines with overexpressed and knocked-out OsGLYI3 exhibited higher and lower germination, respectively. The AA treatment also increased the superoxide dismutase (SOD) activity in the overexpressed transgenic seeds compared to the wild-type seeds yet lowered the SOD activity in the CRISPR/Cas9-derived transgenic rice lines. Rice OsGLYI3 was markedly upregulated in response to NaCl induced stress conditions. Compared to wild-type plants, overexpressed transgenic rice lines exhibited increased GLYI activity, decreased MG levels and improved salt stress tolerance, while CRISPR/Cas9 knockout transgenic rice lines showed decreased glyoxalase I activity, increased MG levels, and greater sensitivity to stress treatments with NaCl. Collectively, our results confirmed for the first time that OsGLYI3 is specifically expressed in rice seeds and contributes to seed longevity and salt stress tolerance.
关键词	Glyoxalase I Methylglyoxal Stress tolerance Seed longevity Rice(Oryza sativa L )
学科领域	Plant Sciences
DOI	10.1016/j.plaphy.2022.04.028
收录类别	SCI
语种	英语
WOS关键词	ORYZA-SATIVA ; I GENE ; METHYLGLYOXAL ; OVEREXPRESSION ; PROTEIN ; IDENTIFICATION ; DETOXIFICATION ; DETERIORATION ; GERMINATION ; DORMANCY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000802221400003
出版者	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
文献子类	Article
出版地	ISSY-LES-MOULINEAUX
EISSN	1873-2690
资助机构	National Natural Science Foundation, China [31871716] ; Science and Technology Program of Guangdong Province, China [2020B121201008, 2020B020209003, 2018B020202004] ; Science and Technology Program of Guangzhou [201909020001, 201807010114] ; Guangdong regional joint fund for basic and applied basic research-key project [2019B1515120061]
作者邮箱	liujun@gdaas.cn ; xiaoyh@hunau.edu.cn
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28678
专题	中科院北方资源植物重点实验室
作者单位	1.Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China 2.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Key Lab Crop Germplasm Resources Preserv, Guangzhou 510640, Peoples R China 3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Liu, Shengjie,Liu, Wenhua,Lai, Jianyun,et al. OsGLYI3, a glyoxalase gene expressed in rice seed, contributes to seed longevity and salt stress tolerance[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2022,183:85-95.
APA	Liu, Shengjie.,Liu, Wenhua.,Lai, Jianyun.,Liu, Qinjian.,Zhang, Wenhu.,...&Xiao, Yinghui.(2022).OsGLYI3, a glyoxalase gene expressed in rice seed, contributes to seed longevity and salt stress tolerance.PLANT PHYSIOLOGY AND BIOCHEMISTRY,183,85-95.
MLA	Liu, Shengjie,et al."OsGLYI3, a glyoxalase gene expressed in rice seed, contributes to seed longevity and salt stress tolerance".PLANT PHYSIOLOGY AND BIOCHEMISTRY 183(2022):85-95.

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