Molecular basis for turnover inefficiencies (misses) during water oxidation in photosystem II

doi:10.1039/d2sc00854h

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	Molecular basis for turnover inefficiencies (misses) during water oxidation in photosystem II
	Han, Guangye 2; Chernev, Petko ; Styring, Stenbjorn ; Messinger, Johannes 1; Mamedov, Fikret
	2022
发表期刊	CHEMICAL SCIENCE
ISSN	2041-6520
卷号	13 期号:29 页码:8667-8678
摘要	Photosynthesis stores solar light as chemical energy and efficiency of this process isv highly important. The electrons required for CO2 reduction are extracted from water in a reaction driven by light-induced charge separations in the Photosystem II reaction center and catalyzed by the CaMn4O5-cluster. This cyclic process involves five redox intermediates known as the S-0-S-4 states. In this study, we quantify the flash-induced turnover efficiency of each S state by electron paramagnetic resonance spectroscopy. Measurements were performed in photosystem II membrane preparations from spinach in the presence of an exogenous electron acceptor at selected temperatures between -10 degrees C and +20 degrees C and at flash frequencies of 1.25, 5 and 10 Hz. The results show that at optimal conditions the turnover efficiencies are limited by reactions occurring in the water oxidizing complex, allowing the extraction of their S state dependence and correlating low efficiencies to structural changes and chemical events during the reaction cycle. At temperatures 10 degrees C and below, the highest efficiency (i.e. lowest miss parameter) was found for the S-1 -> S-2 transition, while the S-2 -> S-3 transition was least efficient (highest miss parameter) over the whole temperature range. These electron paramagnetic resonance results were confirmed by measurements of flash-induced oxygen release patterns in thylakoid membranes and are explained on the basis of S state dependent structural changes at the CaMn4O5-cluster that were determined recently by femtosecond X-ray crystallography. Thereby, possible molecular errors connected to the e(-) transfer, H+ transfer, H2O binding and O-2 release are identified.
学科领域	Chemistry, Multidisciplinary
DOI	10.1039/d2sc00854h
收录类别	SCI
语种	英语
WOS关键词	OXYGEN-EVOLVING COMPLEX ; RAY-ABSORPTION SPECTROSCOPY ; S-STATE TRANSITIONS ; PHOTOSYNTHETIC O2 EVOLUTION ; SP PCC 6803 ; MANGANESE COMPLEX ; P680(+CENTER-DOT) REDUCTION ; TEMPERATURE-DEPENDENCE ; CENTERED OXIDATION ; STRUCTURAL-CHANGES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000823810200001
出版者	ROYAL SOC CHEMISTRY
文献子类	Article
出版地	CAMBRIDGE
EISSN	2041-6539
资助机构	Swedish Research Council [2020-03809] ; NordForsk [82845] ; Swedish Research Council [2020-03809] Funding Source: Swedish Research Council ; NordForsk [82845] Funding Source: researchfish
作者邮箱	fikret.mamedov@kemi.uu.se
作品OA属性	gold, Green Published
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28681
专题	中科院光生物学重点实验室
作者单位	1.Uppsala Univ, Dept Chem, Angstrom Lab, Mol Biomimet, Box 523, S-75120 Uppsala, Sweden 2.Umea Univ, Dept Chem, S-90187 Umea, Sweden 3.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, 20 Nanxincun, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Han, Guangye,Chernev, Petko,Styring, Stenbjorn,et al. Molecular basis for turnover inefficiencies (misses) during water oxidation in photosystem II[J]. CHEMICAL SCIENCE,2022,13(29):8667-8678.
APA	Han, Guangye,Chernev, Petko,Styring, Stenbjorn,Messinger, Johannes,&Mamedov, Fikret.(2022).Molecular basis for turnover inefficiencies (misses) during water oxidation in photosystem II.CHEMICAL SCIENCE,13(29),8667-8678.
MLA	Han, Guangye,et al."Molecular basis for turnover inefficiencies (misses) during water oxidation in photosystem II".CHEMICAL SCIENCE 13.29(2022):8667-8678.

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