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| Genome-Resolved Metagenomics Reveals Distinct Phosphorus Acquisition Strategies between Soil Microbiomes | |
| Wu, Xingjie1; Rensing, Christopher2; Han, Dongfei3; Xiao, Ke-Qing; Dai, Yuexiu1; Tang, Zhixi5; Liesack, Werner6; Peng, Jingjing1; Cui, Zhenling1; Zhang, Fusuo1 | |
| 2022 | |
| 发表期刊 | MSYSTEMS
 |
| ISSN | 2379-5077 |
| 卷号 | 7期号:1 |
| 摘要 | Enhancing soil phosphate solubilization is a promising strategy for agricultural sustainability, while little is known about the mechanisms of how microorganisms cope with differing phosphorus availability. Using a combination of genome-resolved metagenomics and amplicon sequencing, we investigated the microbial mechanisms involved in phosphorus cycling under three agricultural treatments in a wheat-maize rotation system and two natural reforestation treatments. Available soil phosphorus was the key factor shaping bacterial and fungal community composition and function across our agricultural and reforestation sites. Membrane-bound quinoprotein glucose dehydrogenase (PQQGDH) and exopolyphosphatases (PPX) governed microbial phosphate solubilization in agroecosystems. In contrast, genes encoding glycerol-3-phosphate transporters (ugpB, ugpC, and ugpQ) displayed a significantly greater abundance in the reforestation soils. The gcd gene encoding PQQGDH was found to be the best determinant for bioavailable soil phosphorus. Metagenome-assembled genomes (MAGs) affiliated with Cyclobacteriaceae and Vicinamibacterales were obtained from agricultural soils. Their MAGs harbored not only gcd but also the pit gene encoding low-affinity phos-phate transporters. MAGs obtained from reforestation soils were affiliated with Microtrichales and Burkholderiales. These contain ugp genes but no gcd, and thereby are indicative of a phosphate transporter strategy. Our study demonstrates that knowledge of distinct microbial phosphorus acquisition strategies between agricultural and reforestation soils could help in linking microbial processes with phosphorus cycling. IMPORTANCE The soil microbiome is the key player regulating phosphorus cycling proc-esses. Identifying phosphate-solubilizing bacteria and utilizing them for release of recal-citrant phosphate that is bound to rocks or minerals have implications for improving crop nutrient acquisition and crop productivity. In this study, we combined functional metagenomics and amplicon sequencing to analyze microbial phosphorus cycling proc-esses in natural reforestation and agricultural soils. We found that the phosphorus ac-quisition strategies significantly differed between these two ecosystems. A microbial phosphorus solubilization strategy dominated in the agricultural soils, while a microbial phosphate transporter strategy was observed in the reforestation soils. We further iden-tified microbial taxa that contributed to enhanced phosphate solubilization in the agro-ecosystem. These microbes are predicted to be beneficial for the increase in phosphate bioavailability through agricultural practices. |
| 关键词 | phosphorus gcd genome microbiome metagenomics MAGs phosphorous |
| 学科领域 | Microbiology |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | PHOSPHATE SOLUBILIZATION ; FERTILIZATION ; GENETICS ; SEQUENCE ; NITROGEN ; PLANT |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000760768900010 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 文献子类 | Article |
| 出版地 | WASHINGTON |
| 资助机构 | Research and Application of Key Technologies for Soil Quality Improvement and Agricultural Green Development [Z191100004019013] ; National Key Research and Development Program of China [2021YFD1900100] ; National Natural Science Foundation of China [41977038] ; 2115 Talent Development Program of China Agricultural University |
| 作者邮箱 | jingjing.peng@cau.edu.cn |
| 作品OA属性 | Green Published |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/28728 |
| 专题 | 植被与环境变化国家重点实验室 |
| 作者单位 | 1.China Agr Univ, Natl Acad Agr Green Dev, Coll Resources & Environm Sci, Key Lab Plant Soil Interact, Beijing, Peoples R China 2.Natl Observat & Res Stn Agr Green Dev, Quzhou, Hebei, Peoples R China 3.Fujian Agr & Forestry Univ, Coll Resource & Environm, Inst Environm Microbiol, Fuzhou, Peoples R China 4.Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Beijing, Peoples R China 5.Univ Leeds, Sch Earth & Environm, Leeds, W Yorkshire, England 6.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing, Peoples R China 7.Max Planck Inst Terr Microbiol, Res Grp Methanotroph Bacteria & Environm Genom Tr, Marburg, Germany |
| 推荐引用方式 GB/T 7714 | Wu, Xingjie,Rensing, Christopher,Han, Dongfei,et al. Genome-Resolved Metagenomics Reveals Distinct Phosphorus Acquisition Strategies between Soil Microbiomes[J]. MSYSTEMS,2022,7(1). |
| APA | Wu, Xingjie.,Rensing, Christopher.,Han, Dongfei.,Xiao, Ke-Qing.,Dai, Yuexiu.,...&Zhang, Fusuo.(2022).Genome-Resolved Metagenomics Reveals Distinct Phosphorus Acquisition Strategies between Soil Microbiomes.MSYSTEMS,7(1). |
| MLA | Wu, Xingjie,et al."Genome-Resolved Metagenomics Reveals Distinct Phosphorus Acquisition Strategies between Soil Microbiomes".MSYSTEMS 7.1(2022). |
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| wu-et-al-2022-genome（3119KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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