Transcriptomic and physio-biochemical features in rice (Oryza sativa L.) in response to mercury stress

doi:10.1016/j.chemosphere.2022.136612

	Transcriptomic and physio-biochemical features in rice (Oryza sativa L.) in response to mercury stress
	Huang, Yingmei 3,4; Li, Fangbai 3; Yi, Jicai 5; Yan, Huili 6; He, Zhenyan6 ; Li, Xiaomin 1,2
	2022
发表期刊	CHEMOSPHERE
ISSN	0045-6535
卷号	309
摘要	Mercury (Hg) is a toxic and nonessential element for organisms, and its contamination in the environment is a global concern. Previous research has shown that Hg stress may cause severe damage to rice roots; however, the transcriptomic changes in roots and physio-biochemical responses in leaves to different levels of Hg stress are not fully understood. In the present study, rice seedlings were exposed to 20, 80, and 160 mu M HgCl2 for three days in hydroponic experiments. The results showed that the majority of Hg was accumulated in rice roots after Hg exposure, and the 80-and 160-mu M Hg stresses significantly increased the root-to-shoot translocation factors relative to 20-mu M Hg stress, resulting in elevated Hg concentrations in rice shoots. Only the 160-mu M Hg stress significantly inhibited root growth compared with the control, while photosynthesis capacity in leaves was significantly reduced under Hg stress. RNA transcriptome sequencing analyses of the roots showed that common responsive differentially expressed genes were strongly associated with glutathione metabolism, amino acid biosynthesis, and secondary metabolite metabolism, which may play significant roles in Hg accumulation by rice plants. Nine crucial genes identified by protein-protein interaction network analysis may be used as candidate target genes for further investigation of the detoxification mechanism, encoding proteins involved in jasmonic acid synthesis, sugar metabolism, allene oxide synthase, glutathione peroxidase, dismutase, and catalase. Furthermore, physio-biochemical analyses of the leaves indicated that higher production of reactive oxygen species was induced by Hg stress, while glutathione and antioxidant enzymes may play crucial roles in Hg detoxification. Our findings provide transcriptomic and physio-biochemical features of rice roots and shoots, which advance our understanding of the responsive and detoxification mechanisms in rice under different levels of Hg stress.
关键词	Rice leaf Mercury exposure Chlorophyll Differentially expressed gene Glutathione Reactive oxygen species
学科领域	Environmental Sciences
DOI	10.1016/j.chemosphere.2022.136612
收录类别	SCI
语种	英语
WOS关键词	PHENYLALANINE AMMONIA-LYASE ; INORGANIC MERCURY ; TOXICITY ; CADMIUM ; METHYLMERCURY ; TRANSLOCATION ; GLUTATHIONE ; POLLUTION ; PATHWAY ; ROOTS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000933948900003
出版者	PERGAMON-ELSEVIER SCIENCE LTD
文献子类	Article
出版地	OXFORD
EISSN	1879-1298
资助机构	National Natural Sci- ence Foundation of China [42030702] ; Guangdong Key Research and Development Project [2019B110207002] ; Natural Science Foundation of Guangdong Province [2021A1515110574] ; Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program [2017BT01Z176]
作者邮箱	xiaomin.li@m.scnu.edu.cn
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28752
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Guangzhou Inst Geochem, Guangzhou 510640, Peoples R China 2.South China Normal Univ, SCNU Environm Res Inst, Guangdong Prov Key Lab Chem Pollut & Environm Safe, Guangzhou 510006, Peoples R China 3.South China Normal Univ, MOE Key Lab Theoret Chem Environm, Guangzhou 510006, Peoples R China 4.Guangdong Acad Sci, Inst Ecoenvironm & Soil Sci, Natl Reg Joint Engn Res Ctr Soil Pollut Control &, Guangdong Key Lab Integrated Agroenvironm Pollut C, Guangzhou 510650, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.South China Agr Univ, Coll Life Sci, Guangzhou 510642, Peoples R China 7.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Huang, Yingmei,Li, Fangbai,Yi, Jicai,et al. Transcriptomic and physio-biochemical features in rice (Oryza sativa L.) in response to mercury stress[J]. CHEMOSPHERE,2022,309.
APA	Huang, Yingmei,Li, Fangbai,Yi, Jicai,Yan, Huili,He, Zhenyan,&Li, Xiaomin.(2022).Transcriptomic and physio-biochemical features in rice (Oryza sativa L.) in response to mercury stress.CHEMOSPHERE,309.
MLA	Huang, Yingmei,et al."Transcriptomic and physio-biochemical features in rice (Oryza sativa L.) in response to mercury stress".CHEMOSPHERE 309(2022).

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