Asymmetric structure of the native Rhodobacter sphaeroides dimeric LH1-RC complex

doi:10.1038/s41467-022-29453-8

IB-CAS > 中科院光生物学重点实验室

	Asymmetric structure of the native Rhodobacter sphaeroides dimeric LH1-RC complex
	Tani, Kazutoshi ; Kanno, Ryo ; Kikuchi, Riku ; Kawamura, Saki ; Nagashima, Kenji V. P.; Hall, Malgorzata ; Takahashi, Ai ; Yu, Long-Jiang; Kimura, Yukihiro 5; Madigan, Michael T.; Mizoguchi, Akira ; Humbel, Bruno M.; Wang-Otomo, Zheng-Yu
	2022
发表期刊	NATURE COMMUNICATIONS
卷号	13 期号:1
摘要	Rhodobacter sphaeroides is a model organism for studying bacterial photosynthesis. Here, the authors present structures of its native dimeric and a protein-U-lacking monomeric light-harvesting-reaction center complexes, which reveal asymmetric features for the dimer and an altered shape for the monomer. Rhodobacter sphaeroides is a model organism in bacterial photosynthesis, and its light-harvesting-reaction center (LH1-RC) complex contains both dimeric and monomeric forms. Here we present cryo-EM structures of the native LH1-RC dimer and an LH1-RC monomer lacking protein-U (Delta U). The native dimer reveals several asymmetric features including the arrangement of its two monomeric components, the structural integrity of protein-U, the overall organization of LH1, and rigidities of the proteins and pigments. PufX plays a critical role in connecting the two monomers in a dimer, with one PufX interacting at its N-terminus with another PufX and an LH1 beta-polypeptide in the other monomer. One protein-U was only partially resolved in the dimeric structure, signaling different degrees of disorder in the two monomers. The Delta U LH1-RC monomer was half-moon-shaped and contained 11 alpha- and 10 beta-polypeptides, indicating a critical role for protein-U in controlling the number of alpha beta-subunits required for dimer assembly and stabilization. These features are discussed in relation to membrane topology and an assembly model proposed for the native dimeric complex.
学科领域	Multidisciplinary Sciences
DOI	10.1038/s41467-022-29453-8
收录类别	SCI
语种	英语
WOS关键词	PHOTOSYNTHETIC CORE COMPLEX ; RC-LH1-PUFX COMPLEX ; PUFX ; DIMERIZATION ; PROTEIN ; ORGANIZATION ; ARCHITECTURE ; MEMBRANES ; SUBUNIT
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000779784400002
出版者	NATURE PORTFOLIO
文献子类	Article
出版地	BERLIN
EISSN	2041-1723
资助机构	Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED [JP20am0101118, 1758, JP20am0101116, 1878, 17am0101116j0001, 18am0101116j0002, 19am0101116j0003] ; Okinawa Institute of Science and Technology ; Japanese Cabinet Office ; National Key R&D Program of China [2019YFA0904600] ; JSPS KAKENHI [JP16H04174, JP18H05153, JP20H05086, JP20H02856]
作者邮箱	ktani@doc.medic.mie-u.ac.jp ; wang@ml.ibaraki.ac.jp
作品OA属性	Green Submitted, Green Published, gold
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28760
专题	中科院光生物学重点实验室
作者单位	1.Mie Univ, Grad Sch Med, Tsu, Mie 5148507, Japan 2.Kanno, Ryo; Hall, Malgorzata; Takahashi, Ai; Humbel, Bruno M.] Okinawa Inst Sci & Technol Grad Univ OIST, Res Support Div, Imaging Sect, 1919-1 Tancha, Onna, Okinawa 9040495, Japan 3.Ibaraki Univ, Fac Sci, Mito, Ibaraki 3108512, Japan 4.Nagashima, Kenji V. P.] Kanagawa Univ, Res Inst Integrated Sci, 2946 Tsuchiya, Hiratsuka, Kanagawa 2591293, Japan 5.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China 6.Kobe Univ, Grad Sch Agr, Dept Agrobiosci, Nada Ku, Kobe, Hyogo 6578501, Japan 7.Madigan, Michael T.] Southern Illinois Univ, Sch Biol Sci, Dept Microbiol, Carbondale, IL 62901 USA
推荐引用方式 GB/T 7714	Tani, Kazutoshi,Kanno, Ryo,Kikuchi, Riku,et al. Asymmetric structure of the native Rhodobacter sphaeroides dimeric LH1-RC complex[J]. NATURE COMMUNICATIONS,2022,13(1).
APA	Tani, Kazutoshi.,Kanno, Ryo.,Kikuchi, Riku.,Kawamura, Saki.,Nagashima, Kenji V. P..,...&Wang-Otomo, Zheng-Yu.(2022).Asymmetric structure of the native Rhodobacter sphaeroides dimeric LH1-RC complex.NATURE COMMUNICATIONS,13(1).
MLA	Tani, Kazutoshi,et al."Asymmetric structure of the native Rhodobacter sphaeroides dimeric LH1-RC complex".NATURE COMMUNICATIONS 13.1(2022).

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