An LH1-RC photocomplex from an extremophilic phototroph provides insight into origins of two photosynthesis proteins

doi:10.1038/s42003-022-04174-2

IB-CAS > 中科院光生物学重点实验室

	An LH1-RC photocomplex from an extremophilic phototroph provides insight into origins of two photosynthesis proteins
	Tani, Kazutoshi ; Kanno, Ryo ; Kurosawa, Keigo ; Takaichi, Shinichi 3; Nagashima, Kenji V. P.; Hall, Malgorzata ; Yu, Long-Jiang; Kimura, Yukihiro 6; Madigan, Michael T.; Mizoguchi, Akira ; Humbel, Bruno M.; Wang-Otomo, Zheng-Yu
	2022
发表期刊	COMMUNICATIONS BIOLOGY
卷号	5 期号:1
摘要	The cryo-EM structure of the unusual light-harvesting 1-reaction center (LH1-RC) complex from Rpi. globiformis, the most acidophilic anaerobic purple bacteria, is presented, characterized and compared to other photosynthetic bacteria. Rhodopila globiformis is the most acidophilic of anaerobic purple phototrophs, growing optimally in culture at pH 5. Here we present a cryo-EM structure of the light-harvesting 1-reaction center (LH1-RC) complex from Rhodopila globiformis at 2.24 angstrom resolution. All purple bacterial cytochrome (Cyt, encoded by the gene pufC) subunit-associated RCs with known structures have their N-termini truncated. By contrast, the Rhodopila globiformis RC contains a full-length tetra-heme Cyt with its N-terminus embedded in the membrane forming an alpha-helix as the membrane anchor. Comparison of the N-terminal regions of the Cyt with PufX polypeptides widely distributed in Rhodobacter species reveals significant structural similarities, supporting a longstanding hypothesis that PufX is phylogenetically related to the N-terminus of the RC-bound Cyt subunit and that a common ancestor of phototrophic Proteobacteria contained a full-length tetra-heme Cyt subunit that evolved independently through partial deletions of its pufC gene. Eleven copies of a novel gamma-like polypeptide were also identified in the bacteriochlorophyll a-containing Rhodopila globiformis LH1 complex; gamma-polypeptides have previously been found only in the LH1 of bacteriochlorophyll b-containing species. These features are discussed in relation to their predicted functions of stabilizing the LH1 structure and regulating quinone transport under the warm acidic conditions.
学科领域	Biology ; Multidisciplinary Sciences
DOI	10.1038/s42003-022-04174-2
收录类别	SCI
语种	英语
WOS关键词	CRYO-EM STRUCTURE ; RHODOPSEUDOMONAS-VIRIDIS ; CYTOCHROME SUBUNIT ; CORE COMPLEX ; PUF OPERON ; GLOBIFORMIS ; SEQUENCES ; EXPRESSION ; FEATURES ; RUBRUM
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000879788300005
出版者	NATURE PORTFOLIO
文献子类	Article
出版地	BERLIN
EISSN	2399-3642
资助机构	Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED [JP20am0101118, 1758, JP20am0101116, 1878, 17am0101116j0001, 18am0101116j0002, 19am0101116j0003, 22am121004] ; Okinawa Institute of Science and Technology (OIST), Scientific Computing & Data Analysis Section at OIST ; Japanese Cabinet Office ; National Key R&D Program of China [2021YFA0909600] ; JSPS KAKENHI [JP16H04174, JP18H05153, JP20H05086, JP20H02856] ; Takeda Science Foundation ; Kurata Memorial Hitachi Science and Technology Foundation, Japan
作者邮箱	ktani@doc.medic.mie-u.ac.jp ; wang@ml.ibaraki.ac.jp
作品OA属性	Green Published, gold
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28761
专题	中科院光生物学重点实验室
作者单位	1.Mie Univ, Grad Sch Med, Tsu, Mie 5148507, Japan 2.Kanno, Ryo; Hall, Malgorzata; Humbel, Bruno M.] Okinawa Inst Sci & Technol Grad Univ OIST, Res Support Div, Imaging Sect, 1919-1 Tancha, Onna Son, Okinawa 9040495, Japan 3.Ibaraki Univ, Fac Sci, Mito, Ibaraki 3108512, Japan 4.Tokyo Univ Agr, Fac Sci, Dept Mol Microbiol, Setagaya Ku, Tokyo 1568502, Japan 5.Nagashima, Kenji V. P.] Kanagawa Univ, Res Inst Integrated Sci, 2946 Tsuchiya, Hiratsuka, Kanagawa 2591293, Japan 6.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 7.Kobe Univ, Grad Sch Agr, Dept Agrobiosci, Kobe, Hyogo 6578501, Japan 8.Madigan, Michael T.] Southern Illinois Univ, Sch Biol Sci, Dept Microbiol, Carbondale, IL 62901 USA
推荐引用方式 GB/T 7714	Tani, Kazutoshi,Kanno, Ryo,Kurosawa, Keigo,et al. An LH1-RC photocomplex from an extremophilic phototroph provides insight into origins of two photosynthesis proteins[J]. COMMUNICATIONS BIOLOGY,2022,5(1).
APA	Tani, Kazutoshi.,Kanno, Ryo.,Kurosawa, Keigo.,Takaichi, Shinichi.,Nagashima, Kenji V. P..,...&Wang-Otomo, Zheng-Yu.(2022).An LH1-RC photocomplex from an extremophilic phototroph provides insight into origins of two photosynthesis proteins.COMMUNICATIONS BIOLOGY,5(1).
MLA	Tani, Kazutoshi,et al."An LH1-RC photocomplex from an extremophilic phototroph provides insight into origins of two photosynthesis proteins".COMMUNICATIONS BIOLOGY 5.1(2022).

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