OsMADS58 Stabilizes Gene Regulatory Circuits during Rice Stamen Development

doi:10.3390/plants11212899

	OsMADS58 Stabilizes Gene Regulatory Circuits during Rice Stamen Development
	Shen, Liping 1; Tian, Feng 2,3,4; Cheng, Zhukuan 5; Zhao, Qiang 6; Feng, Qi 6; Zhao, Yan 6; Han, Bin 6; Fang, Yuhan ; Lin, Yanan ; Chen, Rui ; Wang, Donghui ; Sun, Wenfeng 7; Sun, Jiaqi 8; Zeng, Hongyun 8; Yao, Nan 8; Gao, Ge 2,3,4; Luo, Jingchu 2,4; Xu, Zhihong ; Bai, Shunong 2,9
	2022
发表期刊	PLANTS-BASEL
卷号	11 期号:21
摘要	Rice (Oryza sativa) OsMADS58 is a C-class MADS box protein, and characterization of a transposon insertion mutant osmads58 suggested that OsMADS58 plays a role in stamen development. However, as no null mutation has been obtained, its role has remained unclear. Here, we report that the CRISPR knockout mutant osmads58 exhibits complex altered phenotypes, including anomalous diploid germ cells, aberrant meiosis, and delayed tapetum degeneration. This CRISPR mutant line exhibited stronger changes in expression of OsMADS58 target genes compared with the osmads58 dSpm (transposon insertion) line, along with changes in multiple pathways related to early stamen development. Notably, transcriptional regulatory circuits in young panicles covering the stamen at stages 4-6 were substantially altered in the CRISPR line compared to the dSpm line. These findings strongly suggest that the pleiotropic effects of OsMADS58 on stamen development derive from a potential role in stabilizing gene regulatory circuits during early stamen development. Thus, this work opens new avenues for viewing and deciphering the regulatory mechanisms of early stamen development from a network perspective.
关键词	OsMADS58 rice stamen meiosis tapetum gene regulatory circuits
学科领域	Plant Sciences
DOI	10.3390/plants11212899
收录类别	SCI
语种	英语
WOS关键词	SYNAPTONEMAL COMPLEX ; MOLECULAR ANALYSIS ; FLOWER DEVELOPMENT ; ARABIDOPSIS ; REVEALS ; ENCODES ; SPOROGENESIS ; INITIATION ; ALIGNMENT ; MERISTEM
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000881681200001
出版者	MDPI
文献子类	Article
出版地	BASEL
EISSN	2223-7747
资助机构	National Natural Science Foundation of China [31630006] ; Ministry of Agriculture of the People's Republic of China [2016ZX08009003-003, CARS-01-06, 2016ZX08010001] ; Ministry of Science and Technology of PRC [2009CB94150]
作者邮箱	shunongb@pku.edu.cn
作品OA属性	gold, Green Submitted, Green Published
引用统计
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28779
专题	中科院植物分子生理学重点实验室
作者单位	1.Peking Univ, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China 4.Peking Univ, Biomed Pioneering Innovat Ctr Biop, Beijing Adv Innovat Ctr Genom ICG, Ctr Bioinformat CBI, Beijing 100871, Peoples R China 5.Peking Univ, Ctr Bioinformat, Beijing 100871, Peoples R China 6.Yangzhou Univ, Jiangsu Coinnovat Ctr Modern Prod Technol Grain C, Yangzhou 225009, Jiangsu, Peoples R China 7.Chinese Acad Sci, Natl Ctr Gene Res, State Key Lab Plant Mol Genet, CAS Ctr Excellence Mol Plant Sci,Inst Plant Physi, Shanghai 200233, Peoples R China 8.Novogene, Beijing 100015, Peoples R China 9.Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510260, Peoples R China 10.Peking Univ, Ctr Quantitat Biol, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Shen, Liping,Tian, Feng,Cheng, Zhukuan,et al. OsMADS58 Stabilizes Gene Regulatory Circuits during Rice Stamen Development[J]. PLANTS-BASEL,2022,11(21).
APA	Shen, Liping.,Tian, Feng.,Cheng, Zhukuan.,Zhao, Qiang.,Feng, Qi.,...&Bai, Shunong.(2022).OsMADS58 Stabilizes Gene Regulatory Circuits during Rice Stamen Development.PLANTS-BASEL,11(21).
MLA	Shen, Liping,et al."OsMADS58 Stabilizes Gene Regulatory Circuits during Rice Stamen Development".PLANTS-BASEL 11.21(2022).

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