Installing a Green Engine To Drive an Enzyme Cascade: A Light-Powered In Vitro Biosystem for Poly(3-hydroxybutyrate) Synthesis

doi:10.1002/anie.202111054

	Installing a Green Engine To Drive an Enzyme Cascade: A Light-Powered In Vitro Biosystem for Poly(3-hydroxybutyrate) Synthesis
	Li, Fei ; Wei, Xinlei ; Zhang, Lin 1; Liu, Cheng 1; You, Chun 2,3; Zhu, Zhiguang 2,3
	2022
发表期刊	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
ISSN	1433-7851
卷号	61 期号:1
摘要	Many existing in vitro biosystems harness power from the chemical energy contained in substrates and co-substrates, and light or electric energy provided from abiotic parts, leading to a compromise in atom economy, incompatibility between biological and abiotic parts, and most importantly, incapability to spatiotemporally co-regenerate ATP and NADPH. In this study, we developed a light-powered in vitro biosystem for poly(3-hydroxybutyrate) (PHB) synthesis using natural thylakoid membranes (TMs) to regenerate ATP and NADPH for a five-enzyme cascade. Through effective coupling of cofactor regeneration and mass conversion, 20 mM PHB was yielded from 50 mM sodium acetate with a molar conversion efficiency of carbon of 80.0 % and a light-energy conversion efficiency of 3.04 %, which are much higher than the efficiencies of similar in vitro PHB synthesis biosystems. This suggests the promise of installing TMs as a green engine to drive more enzyme cascades.
关键词	enzyme cascades in vitro synthetic biosystems light energy conversion polyhydroxybutyrate thylakoid membranes
学科领域	Chemistry, Multidisciplinary
DOI	10.1002/anie.202111054
收录类别	SCI
语种	英语
WOS关键词	THYLAKOID MEMBRANES ; PHOTOSYNTHESIS ; GLYCINEBETAINE ; BIOCATALYSIS ; PHOTOCURRENT ; BIOCHEMISTRY ; BIOMASS ; ATP
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000720982600001
出版者	WILEY-V C H VERLAG GMBH
文献子类	Article
出版地	WEINHEIM
EISSN	1521-3773
资助机构	Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project [TSBICIP-KJGG-007-13] ; CAS Pioneer Hundred Talents Program [2016-081] ; Postdoctoral Program of Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project [TSBICIP-CXRC-054] ; National Natural Science Foundation of China [32101167]
作者邮箱	zhu_zg@tib.cas.cn
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28786
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, 32 West 7th Ave, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 4.Natl Technol Innovat Ctr Synthet Biol, 32 West 7th Ave, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Li, Fei,Wei, Xinlei,Zhang, Lin,et al. Installing a Green Engine To Drive an Enzyme Cascade: A Light-Powered In Vitro Biosystem for Poly(3-hydroxybutyrate) Synthesis[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2022,61(1).
APA	Li, Fei,Wei, Xinlei,Zhang, Lin,Liu, Cheng,You, Chun,&Zhu, Zhiguang.(2022).Installing a Green Engine To Drive an Enzyme Cascade: A Light-Powered In Vitro Biosystem for Poly(3-hydroxybutyrate) Synthesis.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,61(1).
MLA	Li, Fei,et al."Installing a Green Engine To Drive an Enzyme Cascade: A Light-Powered In Vitro Biosystem for Poly(3-hydroxybutyrate) Synthesis".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 61.1(2022).

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