Overexpression of grape ABA receptor gene VaPYL4 enhances tolerance to multiple abiotic stresses in Arabidopsis

doi:10.1186/s12870-022-03663-0

	Overexpression of grape ABA receptor gene VaPYL4 enhances tolerance to multiple abiotic stresses in Arabidopsis
	Ren, Chong ; Kuang, Yangfu 1; Lin, Yanping 1; Guo, Yuchen 1; Li, Huayang 1; Fan, Peige ; Li, Shaohua; Liang, Zhenchang
	2022
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	22 期号:1
摘要	Background Abscisic acid (ABA) plays a crucial role in abiotic stress responses. The pyrabactin resistance (PYR)/PYR-like (PYL)/regulatory component of ABA receptor (RCAR) proteins that have been characterized as ABA receptors function as the core components in ABA signaling pathway. However, the functions of grape PYL genes in response to different abiotic stresses, particularly cold stress, remain less studied. Results In this study, we investigated the expression profiles of grape PYL genes upon cold treatment and isolated the VaPYL4 gene from Vitis amurensis, a cold-hardy grape species. Overexpression of VaPYL4 gene in grape calli and Arabidopsis resulted in enhanced cold tolerance. Moreover, plant resistance to drought and salt stress was also improved by overexpressing VaPYL4 in Arabidopsis. More importantly, we evaluated the contribution of VaPYL4 to plant growth and development after the treatment with cold, salt and drought stress simultaneously. The transgenic plants showed higher survival rates, earlier flowering phenotype, and heavier fresh weight of seedlings and siliques when compared with wild-type plants. Physiological analyses showed that transgenic plants had much lower content of malondialdehyde (MDA) and higher peroxidase (POD) activity. Stress-responsive genes such as RD29A (Responsive to desiccation 29A), COR15A (Cold responsive 15A) and KIN2 (Kinase 2) were also significantly up-regulated in VaPYL4-overexpressing Arabidopsis plants. Conclusions Our results show that overexpression of VaPYL4 could improve plant performance upon different abiotic stresses, which therefore provides a useful strategy for engineering future crops to deal with adverse environments.
关键词	Abscisic acid Vitis amurensis VaPYL4 Overexpression Abiotic stress
学科领域	Plant Sciences
DOI	10.1186/s12870-022-03663-0
收录类别	SCI
语种	英语
WOS关键词	ABSCISIC-ACID ; SIGNAL-TRANSDUCTION ; DROUGHT TOLERANCE ; FAMILY ; COLD ; EXPRESSION ; STRATEGIES ; COMPONENTS ; RESISTANCE ; AREB1
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000805191600001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	National Natural Science Foundation of China [32001994, 32025032] ; National Key Research and Development Program of China [2019YFD1002501] ; Agricultural Breeding Project of Ningxia Hui Autonomous Region [NXNYYZ20210104]
作者邮箱	zl249@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28791
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, Key Lab Plant Resource, Nanxin Village 20, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ren, Chong,Kuang, Yangfu,Lin, Yanping,et al. Overexpression of grape ABA receptor gene VaPYL4 enhances tolerance to multiple abiotic stresses in Arabidopsis[J]. BMC PLANT BIOLOGY,2022,22(1).
APA	Ren, Chong.,Kuang, Yangfu.,Lin, Yanping.,Guo, Yuchen.,Li, Huayang.,...&Liang, Zhenchang.(2022).Overexpression of grape ABA receptor gene VaPYL4 enhances tolerance to multiple abiotic stresses in Arabidopsis.BMC PLANT BIOLOGY,22(1).
MLA	Ren, Chong,et al."Overexpression of grape ABA receptor gene VaPYL4 enhances tolerance to multiple abiotic stresses in Arabidopsis".BMC PLANT BIOLOGY 22.1(2022).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Ren-2022-Overexpress（5698KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文