Photoprotective energy quenching in the red alga Porphyridium purpureum occurs at the core antenna of the photosystem II but not at its reaction center

doi:10.1016/j.jbc.2022.101783

IB-CAS > 中科院光生物学重点实验室

	Photoprotective energy quenching in the red alga Porphyridium purpureum occurs at the core antenna of the photosystem II but not at its reaction center
	Fang, Yuan 1; Liu, Dongyang 1; Jiang, Jingjing ; He, Axin 2; Zhu, Rui 3; Tian, Lijin
	2022
发表期刊	JOURNAL OF BIOLOGICAL CHEMISTRY
卷号	298 期号:4
摘要	Photosynthetic organisms have evolved light-harvesting antennae over time. In cyanobacteria, external phycobilisomes (PBSs) are the dominant antennae, whereas in green algae and higher plants, PBSs have been replaced by proteins of the Lhc family that are integrated in the membrane. Red algae represent an evolutionary intermediate between these two systems, as they employ both PBSs and membrane LHCR proteins as light-harvesting units. Understanding how red algae cope with light is not only interesting for biotechnological applications, but is also of evolutionary interest. For example, energy-dependent quenching (qE) is an essential photoprotective mechanism widely used by species from cyanobacteria to higher plants to avoid light damage; however, the quenching mechanism in red algae remains largely unexplored. Here, we used both pulse amplitude-modulated (PAM) and time-resolved chlorophyll fluorescence to characterize qE kinetics in the red alga Porphyridium purpureum. PAM traces confirmed that qE in P. purpureum is activated by a decrease in the thylakoid lumen pH, whereas time-resolved fluorescence results further revealed the quenching site and ultrafast quenching kinetics. We found that quenching exclusively takes place in the photosystem II (PSII) complexes and preferentially occurs at PSII's core antenna rather than at its reaction center, with an overall quenching rate of 17.6 +/- 3.0 ns(-1). In conclusion, we propose that qE in red algae is not a reaction center type of quenching, and that there might be a membrane-bound protein that resembles PsbS of higher plants or LHCSR of green algae that senses low luminal pH and triggers qE in red algae.
学科领域	Biochemistry & Molecular Biology
DOI	10.1016/j.jbc.2022.101783
收录类别	SCI
语种	英语
WOS关键词	LIGHT-HARVESTING COMPLEXES ; CHLOROPHYLL FLUORESCENCE ; CHLAMYDOMONAS-REINHARDTII ; STATE TRANSITIONS ; EXCESS LIGHT ; PROTEIN ; DISSIPATION ; CYANOBACTERIAL ; MECHANISMS ; MEMBRANES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000823106100009
出版者	ELSEVIER
文献子类	Article
出版地	AMSTERDAM
EISSN	1083-351X
资助机构	National Key Research and Development Program of China [2019YFA0904604] ; National Natural Science Foundation of China [11804172, 31970381, 11605001, 91850104]
作者邮箱	ltian@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28803
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Peking Univ, Sch Phys, State Key Lab Artificial Microstruct & Mesoscop P, Beijing, Peoples R China 4.Peking Univ, Sch Phys, Electron Microscopy Lab, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Fang, Yuan,Liu, Dongyang,Jiang, Jingjing,et al. Photoprotective energy quenching in the red alga Porphyridium purpureum occurs at the core antenna of the photosystem II but not at its reaction center[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2022,298(4).
APA	Fang, Yuan,Liu, Dongyang,Jiang, Jingjing,He, Axin,Zhu, Rui,&Tian, Lijin.(2022).Photoprotective energy quenching in the red alga Porphyridium purpureum occurs at the core antenna of the photosystem II but not at its reaction center.JOURNAL OF BIOLOGICAL CHEMISTRY,298(4).
MLA	Fang, Yuan,et al."Photoprotective energy quenching in the red alga Porphyridium purpureum occurs at the core antenna of the photosystem II but not at its reaction center".JOURNAL OF BIOLOGICAL CHEMISTRY 298.4(2022).

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