Oxidosqualene Cyclases Involved in the Biosynthesis of Diverse Triterpenes in Camellia sasanqua

doi:10.1021/acs.jafc.2c03011

	Oxidosqualene Cyclases Involved in the Biosynthesis of Diverse Triterpenes in Camellia sasanqua
	Huang, Liufang ; Hu, Yonger ; Huang, Ruoshi ; Chen, Jiabo ; Zhang, Xiande ; Yue, Jingyang ; Feng, Laibao 1; She, Yaru ; Ji, Aijia ; Zheng, Ying 2; Liu, Zhongqiu ; Zhang, Rongrong ; Duan, Lixin
	2022
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN	0021-8561
摘要	Camellia sasanqua is an important economic plant that is rich in lipophilic triterpenols with pharmacological activities including antiallergic, anti-inflammatory, and anticancer activities. However, the key enzymes related to triterpene biosynthesis have seldom been studied in C. sasanqua. Oxidosqualene cyclases (OSCs) are the rate-limiting enzymes related to triterpene biosynthesis. In this study, seven putative OSC genes (CsOSC1-7) were mined from the C. sasanqua transcriptome. Six CsOSCs were characterized for the biosynthesis of diverse triterpene skeletons, including alpha-amyrin, beta-amyrin, delta-amyrin, dammarenediol-II, psi taraxasterol, taraxasterol, and cycloartenol by the heterologous expression system. CsOSC3 was a multiple functional alpha-amyrin synthase. Three key residues, Trp260, Tyr262, and Phe415, are critical to the catalytic performance of CsOSC3 judging from the results of molecular docking and site-directed mutagenesis. These findings provide important insights into the biosynthesis pathway of triterpenes in C. sasanqua.
关键词	oxidosqualene cyclases (OSCs) Camellia sasanqua triterpene biosynthesis catalytic mechanism
学科领域	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
DOI	10.1021/acs.jafc.2c03011
收录类别	SCI
语种	英语
WOS关键词	FLOWER BUDS ; SYNTHASE ; ALCOHOLS ; EXPRESSION ; PLANT ; OILS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000821821000001
出版者	AMER CHEMICAL SOC
文献子类	Article; Early Access
出版地	WASHINGTON
EISSN	1520-5118
作者邮箱	liuzq@gzucm.edu.cn ; zrr586@gzucm.edu.cn ; nlizn@gzucm.edu.cn
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28807
专题	中科院植物分子生理学重点实验室
作者单位	1.Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, Int Inst Translat Chinese Med, Joint Lab Translat Canc Res Chinese Med,Minist Ed, Guangzhou 510006, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Guangzhou Univ Chinese Med, Basic Med Sci Coll, Res Ctr Basic Integrat Med, Guangzhou 510006, Peoples R China
推荐引用方式 GB/T 7714	Huang, Liufang,Hu, Yonger,Huang, Ruoshi,et al. Oxidosqualene Cyclases Involved in the Biosynthesis of Diverse Triterpenes in Camellia sasanqua[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2022.
APA	Huang, Liufang.,Hu, Yonger.,Huang, Ruoshi.,Chen, Jiabo.,Zhang, Xiande.,...&Duan, Lixin.(2022).Oxidosqualene Cyclases Involved in the Biosynthesis of Diverse Triterpenes in Camellia sasanqua.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY.
MLA	Huang, Liufang,et al."Oxidosqualene Cyclases Involved in the Biosynthesis of Diverse Triterpenes in Camellia sasanqua".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022).

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