Inhibitory Effect of Acer truncatum Bunge Seed Coat Extract on Fatty Acid Synthase, Differentiation and Lipid Accumulation in 3T3-L1 Adipocytes

doi:10.3390/molecules27041324

	Inhibitory Effect of Acer truncatum Bunge Seed Coat Extract on Fatty Acid Synthase, Differentiation and Lipid Accumulation in 3T3-L1 Adipocytes
	Liang, Yan 1,2; Kong, Fan 1,3; Ma, Xiaofeng 3; Shu, Qingyan 1
	2022
发表期刊	MOLECULES
卷号	27 期号:4
摘要	Acer truncatum Bunge is now widely cultivated throughout the world. Fatty acid synthase (FAS) is a potential target in the treatment of both obesity and cancer. Only a few FAS inhibitors have been reported. In this study, the inhibitory effect of A. truncatum seed coat (ESA) on FAS and the inhibition mechanisms were investigated using a FAS activity assay and an enzyme kinetics study. The main chemicals of ESA were analyzed with UPLC-MS/MS. The effects of ESA on 3T3-L1 adipocyte differentiation and lipid accumulation were investigated using Oil red O staining. We first identified seven main compounds (quinic acid, malic acid, gentisic acid, procyanidin dimer, procyanidin trimer, catechin, and quercetin) from 50% ethanol extracts of seed coats of A. truncatum (ESAs), which were then found to inhibit 3T3-L1 adipocyte differentiation at the concentration of 50 mu g/mL. ESA obviously reduced the visible triglyceride droplets accumulation, and dramatically decreased the number of the adipocytes at a comparatively high concentration. It is suggested that the effects are due to the inhibition of FAS by ESA; FAS activity is inhibited by ESA at a half inhibition concentration (IC50) of 0.57 mu g/mL, which is lower than that of classically known FAS inhibitors. Meanwhile, ESA displayed different inhibition kinetics and reacting sites for FAS. These results provide new clues for the development of novel products for obesity treatment and a scientific basis for the full use of byproducts for future industrial production of vegetable oil.
关键词	fatty acid synthase Acer truncatum Bunge inhibitor obesity
学科领域	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
DOI	10.3390/molecules27041324
收录类别	SCI
语种	英语
WOS关键词	CANCER ; LEAVES ; OBESITY ; WEIGHT ; OIL
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000764362100001
出版者	MDPI
文献子类	Article
出版地	BASEL
EISSN	1420-3049
资助机构	Biological Resources Program, Chinese Academy of Sciences [KFJ-BRP-007] ; Fundamental Research Funds for Central Universities [Y95401AXX2] ; Youth Innovation Promotion Association, CAS [2012315] ; Zhongke Ruilaiyin (Beijing) Biotechnology Co., Ltd [2021C037]
作者邮箱	yanliang@cupes.edu.cn ; kongfan20@mails.ucas.ac.cn ; maxiaofeng@ucas.ac.cn ; shuqy@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28836
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 3.Capital Univ Phys Educ & Sports, Sch Kinesiol & Hlth, 11 Beisanhuanxi Rd, Beijing 100191, Peoples R China 4.Univ Chinese Acad Sci, Coll Life Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liang, Yan,Kong, Fan,Ma, Xiaofeng,et al. Inhibitory Effect of Acer truncatum Bunge Seed Coat Extract on Fatty Acid Synthase, Differentiation and Lipid Accumulation in 3T3-L1 Adipocytes[J]. MOLECULES,2022,27(4).
APA	Liang, Yan,Kong, Fan,Ma, Xiaofeng,&Shu, Qingyan.(2022).Inhibitory Effect of Acer truncatum Bunge Seed Coat Extract on Fatty Acid Synthase, Differentiation and Lipid Accumulation in 3T3-L1 Adipocytes.MOLECULES,27(4).
MLA	Liang, Yan,et al."Inhibitory Effect of Acer truncatum Bunge Seed Coat Extract on Fatty Acid Synthase, Differentiation and Lipid Accumulation in 3T3-L1 Adipocytes".MOLECULES 27.4(2022).

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