Transcriptomic comparison sheds new light on regulatory networks for dimorphic flower development in response to photoperiod in Viola prionantha

doi:10.1186/s12870-022-03732-4

	Transcriptomic comparison sheds new light on regulatory networks for dimorphic flower development in response to photoperiod in Viola prionantha
	Li, Qiaoxia ; Li, Kunpeng 1,2; Zhang, Zhengrong ; Li, Jigang ; Wang, Bo ; Zhang, Zuoming ; Zhu, Yuanyuan ; Pan, Chaochao ; Sun, Kun ; He, Chaoying1,2,3
	2022
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	22 期号:1
摘要	Background Chasmogamous (CH)-cleistogamous (CL) dimorphic flowers are developed in Viola prionantha. However, the environmental and genetic factors necessary for the CH-CL transition are unknown. Results In the present work, short-day (SD) conditions induced CH flowers, whereas long days (LDs) triggered CL flowers in V. prionantha. Compared to fully developed CH flowers, CL flowers had less mature stamens, no nectar glands, and immature petals. Comparative transcriptomics revealed differentially expressed genes (DEGs) during CL and CH development. Core genes in the photoperiod pathway, such as V. prionantha orthologs of GIGANTEA (GI), CONSTANS (CO), and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1), which promote floral induction, were highly expressed in CL flowers, whereas UNUSUAL FLORAL ORGANS (UFO) and B-class MADS-box genes for floral organ identity and development showed an opposite alteration. Moreover, genes in the glycolytic process, sucrose metabolic process, and fatty acid biosynthetic process were all highly expressed in CH flowers. Interestingly, V. prionantha orthologs of the B-class MADS-box genes APETALA3 (AP3) and PISTILLATA (PI) might relate to these sugar-fatty acid processes and were co-expressed with GAIP-B-like and YABBY5 (YAB5), which regulate the development of the petal, stamen, and nectary. Compared to CH flowers, DEGs and hub genes in the most significantly correlated modules of the gene co-expression network, which are involved in abiotic and biotic responses, were upregulated in CL flowers. Conclusions We proposed an integrative model for transcription regulation of genes in the photoperiod pathway, floral organ development, stress response, and sugar-fatty acid processes to determine CH-CL flower development in V. prionantha. Particularly, under LDs, activated GI may induce genes involved in the stress-response pathways, and then downregulated AP3 and PI or UFO to inhibit the sugar-fatty acid metabolic processes, together forming CL flowers. In contrast, CH flowers were produced under SDs. This work provides novel insights into the developmental evolution of dimorphic flowers in Viola.
关键词	Dimorphic flower Differentially expressed genes (DEGs) Photoperiod Floral development Transcriptomics Viola
学科领域	Plant Sciences
DOI	10.1186/s12870-022-03732-4
收录类别	SCI
语种	英语
WOS关键词	ARABIDOPSIS-THALIANA ; FLORAL INDUCTION ; MOLECULAR-BASIS ; ABSCISIC-ACID ; MADS-BOX ; GENE ; CLEISTOGAMY ; TOOL ; BIOSYNTHESIS ; INFORMATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000824929300001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	National Natural Science Foundation of China [32160055, 31930007, 31800188] ; Natural Science Foundation of Gansu province [21JR7RA140]
作者邮箱	liqiaoxia8024@nwnu.edu.cn ; chaoying@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28837
专题	系统与进化植物学国家重点实验室
作者单位	1.Northwest Normal Univ, Life Sci Coll, Anning East Rd 967, Lanzhou 730070, Gansu, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Nanxincun 20, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Qiaoxia,Li, Kunpeng,Zhang, Zhengrong,et al. Transcriptomic comparison sheds new light on regulatory networks for dimorphic flower development in response to photoperiod in Viola prionantha[J]. BMC PLANT BIOLOGY,2022,22(1).
APA	Li, Qiaoxia.,Li, Kunpeng.,Zhang, Zhengrong.,Li, Jigang.,Wang, Bo.,...&He, Chaoying.(2022).Transcriptomic comparison sheds new light on regulatory networks for dimorphic flower development in response to photoperiod in Viola prionantha.BMC PLANT BIOLOGY,22(1).
MLA	Li, Qiaoxia,et al."Transcriptomic comparison sheds new light on regulatory networks for dimorphic flower development in response to photoperiod in Viola prionantha".BMC PLANT BIOLOGY 22.1(2022).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Li-2022-Transcriptom（4574KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文