The genome of Dioscorea zingiberensis sheds light on the biosynthesis, origin and evolution of the medicinally important diosgenin saponins

doi:10.1093/hr/uhac165

	The genome of Dioscorea zingiberensis sheds light on the biosynthesis, origin and evolution of the medicinally important diosgenin saponins
	Li, Yi ; Tan, Chao ; Li, Zihao ; Guo, Jingzhe ; Li, Song ; Chen, Xin ; Wang, Chen ; Dai, Xiaokang ; Yang, Huan ; Song, Wei ; Hou, Lixiu ; Xu, Jiali ; Tong, Ziyu ; Xu, Anran ; Yuan, Xincheng ; Wang, Weipeng ; Yang, Qingyong 1; Chen, Lingling 2; Sun, Zongyi 3; Wang, Kai 3; Pan, Bo 4; Chen, Jianghua 5; Bao, Yinghua 6; Liu, Faguang 6; Qi, Xiaoquan7 ; Gang, David R.; Wen, Jun 9; Li, Jiaru
	2022
发表期刊	HORTICULTURE RESEARCH
ISSN	2662-6810
卷号	9
摘要	Diosgenin saponins isolated from Dioscorea species such as D. zingiberensis exhibit a broad spectrum of pharmacological activities. Diosgenin, the aglycone of diosgenin saponins, is an important starting material for the production of steroidal drugs. However, how plants produce diosgenin saponins and the origin and evolution of the diosgenin saponin biosynthetic pathway remain a mystery. Here we report a high-quality, 629-Mb genome of D. zingiberensis anchored on 10 chromosomes with 30322 protein-coding genes. We reveal that diosgenin is synthesized in leaves ('source'), then converted into diosgenin saponins, and finally transported to rhizomes ('sink') for storage in plants. By evaluating the distribution and evolutionary patterns of diosgenin saponins in Dioscorea species, we find that diosgenin saponin-containing may be an ancestral trait in Dioscorea and is selectively retained. The results of comparative genomic analysis indicate that tandem duplication coupled with a whole-genome duplication event provided key evolutionary resources for the diosgenin saponin biosynthetic pathway in the D. zingiberensis genome. Furthermore, comparative transcriptome and metabolite analysis among 13 Dioscorea species suggests that specific gene expression patterns of pathway genes promote the differential evolution of the diosgenin saponin biosynthetic pathway in Dioscorea species. Our study provides important insights and valuable resources for further understanding the biosynthesis, evolution, and utilization of plant specialized metabolites such as diosgenin saponins.
学科领域	Plant Sciences ; Genetics & Heredity ; Horticulture
DOI	10.1093/hr/uhac165
收录类别	SCI
语种	英语
WOS关键词	STEROIDAL SAPONINS ; PHYLOGENETIC ANALYSIS ; PROVIDES INSIGHTS ; TOOL ; IDENTIFICATION ; PLANTS ; DIVERSITY ; SEQUENCE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000922866000015
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	2052-7276
资助机构	National Natural Science Foundation of China [30370152, 31270345, 31470388] ; USDA National Institute of Food and Agriculture [227700]
作者邮箱	jrli@whu.edu.cn
作品OA属性	Green Published, gold
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28838
专题	中科院植物分子生理学重点实验室
作者单位	1.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China 2.Huazhong Agr Univ, Coll Informat, Hubei Key Lab Agr Bioinformat, Wuhan 430070, Peoples R China 3.Guangxi Univ, Coll Life Sci & Technol, Nanning 530004, Peoples R China 4.Grand Biosci, Beijing 102200, Peoples R China 5.Chinese Acad Sci, Ctr Integrat Conservat, Xishuangbanna Trop Bot Garden, Menglun 666303, Peoples R China 6.CAS Ctr Excellence Mol Plant Sci, CAS Key Lab Trop Plant Resources & Sustainable Us, Xishuangbanna Trop Bot Garden, Kunming 650223, Yunnan, Peoples R China 7.Shaoguan Univ, Henry Fok Coll Biol & Agr, Shaoguan 512005, Peoples R China 8.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 9.Gang, David R.] Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA 10.Smithsonian Inst, Dept Bot, Natl Museum Nat Hist, Washington, DC 20013 USA
推荐引用方式 GB/T 7714	Li, Yi,Tan, Chao,Li, Zihao,et al. The genome of Dioscorea zingiberensis sheds light on the biosynthesis, origin and evolution of the medicinally important diosgenin saponins[J]. HORTICULTURE RESEARCH,2022,9.
APA	Li, Yi.,Tan, Chao.,Li, Zihao.,Guo, Jingzhe.,Li, Song.,...&Li, Jiaru.(2022).The genome of Dioscorea zingiberensis sheds light on the biosynthesis, origin and evolution of the medicinally important diosgenin saponins.HORTICULTURE RESEARCH,9.
MLA	Li, Yi,et al."The genome of Dioscorea zingiberensis sheds light on the biosynthesis, origin and evolution of the medicinally important diosgenin saponins".HORTICULTURE RESEARCH 9(2022).

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