PhUGT78A22, a novel glycosyltransferase in Paeonia 'He Xie', can catalyze the transfer of glucose to glucosylated anthocyanins during petal blotch formation

doi:10.1186/s12870-022-03777-5

	PhUGT78A22, a novel glycosyltransferase in Paeonia 'He Xie', can catalyze the transfer of glucose to glucosylated anthocyanins during petal blotch formation
	Li, Yang ; Kong, Fan 2; Liu, Zheng'an ; Peng, Liping 1; Shu, Qingyan 1
	2022
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	22 期号:1
摘要	Background Flower color patterns play an important role in the evolution and subsequent diversification of flowers by attracting animal pollinators. This interaction can drive the diversity observed in angiosperms today in many plant families such as Liliaceae, Paeoniaceae, and Orchidaceae, and increased their ornamental values. However, the molecular mechanism underlying the differential distribution of anthocyanins within petals remains unclear in Paeonia. Results In this study, we used an intersectional hybrid between the section Moutan and Paeonia, hereafter named Paeonia 'He Xie', which has purple flowers with dark purple blotches. After Ultra-high performance liquid chromatography-diode array detector (UPLC-DAD) analysis of blotched and non-blotched parts of petals, we found the anthocyanin content in the blotched part was always higher than that in the non-blotched part. Four kinds of anthocyanins, namely cyanidin-3-O-glucoside (Cy3G), cyanidin-3,5-O-glucoside (Cy3G5G), peonidin-3-O-glucoside (Pn3G), and peonidin-3,5-O-glucoside (Pn3G5G) were detected in the blotched parts, while only Cy3G5G and Pn3G5G were detected in the non-blotched parts. This suggests that glucosyltransferases may play a vital role in the four kinds of glucosylated anthocyanins in the blotched parts. Moreover, 2433 differentially expressed genes (DEGs) were obtained from transcriptome analysis of blotched and non-blotched parts, and a key UDP-glycosyltransferase named PhUGT78A22 was identified, which could use Cy3G and Pn3G as substrates to produce Cy3G5G and Pn3G5G, respectively, in vitro. Furthermore, silencing of PhUGT78A22 reduced the content of anthocyanidin 3,5-O-diglucoside in P. 'He Xie'. Conclusions A UDP-glycosyltransferase, PhUGT78A22, was identified in P. 'He Xie', and the molecular mechanism underlying differential distribution of anthocyanins within petals was elucidated. This study provides new insights on the biosynthesis of different kinds of anthocyanins within colorful petals, and helps to explain petal blotch formation, which will facilitate the cultivar breeding with respect to increasing ornamental value. Additionally, it provides a reference for understanding the molecular mechanisms responsible for precise regulation of anthocyanin biosynthesis and distribution patterns.
关键词	Anthocyanins di-glucosides UDP-glycosyltransferase Petal blotch formation Paeonia Anthocyanin distribution pattern
学科领域	Plant Sciences
DOI	10.1186/s12870-022-03777-5
收录类别	SCI
语种	英语
WOS关键词	FLOWER COLORATION ; TREE PEONY ; STRESS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000842182800001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	National Natural Science Foundation of China [32072065, 31471909, 2021C072]
作者邮箱	liuzhengan@ibcas.ac.cn ; shuqy@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28839
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Beijing Bot Garden, Inst Bot, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Yang,Kong, Fan,Liu, Zheng'an,et al. PhUGT78A22, a novel glycosyltransferase in Paeonia 'He Xie', can catalyze the transfer of glucose to glucosylated anthocyanins during petal blotch formation[J]. BMC PLANT BIOLOGY,2022,22(1).
APA	Li, Yang,Kong, Fan,Liu, Zheng'an,Peng, Liping,&Shu, Qingyan.(2022).PhUGT78A22, a novel glycosyltransferase in Paeonia 'He Xie', can catalyze the transfer of glucose to glucosylated anthocyanins during petal blotch formation.BMC PLANT BIOLOGY,22(1).
MLA	Li, Yang,et al."PhUGT78A22, a novel glycosyltransferase in Paeonia 'He Xie', can catalyze the transfer of glucose to glucosylated anthocyanins during petal blotch formation".BMC PLANT BIOLOGY 22.1(2022).

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