Identification and Fine Mapping of the Recessive Gene BK-5, Which Affects Cell Wall Biosynthesis and Plant Brittleness in Maize

doi:10.3390/ijms23020814

IB-CAS > 中科院光生物学重点实验室

	Identification and Fine Mapping of the Recessive Gene BK-5, Which Affects Cell Wall Biosynthesis and Plant Brittleness in Maize
	Li, Qigui ; Nie, Shujun ; Li, Gaoke 1; Du, Jiyuan 2; Ren, Ruchang 2; Yang, Xiu 3; Liu, Boyan 2; Gao, Xiaolong 2; Liu, Tianjian 2; Zhang, Zhiming 2; Zhao, Xiangyu 2; Li, Xinzheng 2; Nie, Yongxin 2; Wang, Baichen 3; Lin, Haijian ; Ding, Haiping 2; Pan, Guangtang
	2022
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	23 期号:2
摘要	The cellulose of the plant cell wall indirectly affects the cell shape and straw stiffness of the plant. Here, the novel brittleness mutant brittle stalk-5 (bk-5) of the maize inbred line RP125 was characterized. We found that the mutant displayed brittleness of the stalk and even the whole plant, and that the brittleness phenotype existed during the whole growth period from germination to senescence. The compressive strength was reduced, the cell wall was thinner, and the cellulose content was decreased compared to that of the wild type. Genetic analysis and map-based cloning indicated that bk-5 was controlled by a single recessive nuclear gene and that it was located in a 90.2-Kb region on chromosome 3 that covers three open reading frames (ORFs). Sequence analysis revealed a single non-synonymous missense mutation, T-to-A, in the last exon of Zm00001d043477 (B73: version 4, named BK-5) that caused the 951th amino acid to go from leucine to histidine. BK-5 encodes a cellulose synthase catalytic subunit (CesA), which is involved with cellulose synthesis. We found that BK-5 was constitutively expressed in all tissues of the germinating stage and silking stage, and highly expressed in the leaf, auricula, and root of the silking stage and the 2-cm root and bud of the germinating stage. We found that BK-5 mainly localized to the Golgi apparatus, suggesting that the protein might move to the plasma membrane with the aid of Golgi in maize. According to RNA-seq data, bk-5 had more downregulated genes than upregulated genes, and many of the downregulated genes were enzymes and transcription factors related to cellulose, hemicellulose, and lignin biosynthesis of the secondary cell wall. The other differentially expressed genes were related to metabolic and cellular processes, and were significantly enriched in hormone signal transduction, starch and sucrose metabolism, and the plant-pathogen interaction pathway. Taken together, we propose that the mutation of gene BK-5 causes the brittle stalk phenotype and provides important insights into the regulatory mechanism of cellulose biosynthesis and cell wall development in maize.
关键词	maize brittle stalk cell wall cellulose synthase catalytic subunit (CESA)
学科领域	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
DOI	10.3390/ijms23020814
收录类别	SCI
语种	英语
WOS关键词	CELLULOSE SYNTHASE COMPLEXES ; COBRA-LIKE PROTEIN ; EXPRESSION ANALYSIS ; LODGING RESISTANCE ; CATALYTIC SUBUNIT ; ENCODES ; CESA ; MUTATION ; FAMILY ; ISOXABEN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000748073700001
出版者	MDPI
文献子类	Article
出版地	BASEL
EISSN	1422-0067
资助机构	National Transgenic Major Project of China [2016ZX08003] ; National Natural Science Foundation of China [31771803, 31771802] ; Taishan Scholars program of Shandong Province [tsqn201909074] ; Key projects of Natural Science Foundation of Shandong Province [ZR2020KC019]
作者邮箱	18408211893@163.com ; sjn0923@163.com ; ligaoke790326@163.com ; wsdjy5555555555555@163.com ; sgsiban2010@163.com ; yangxiu@ibcas.ac.cn ; byan_liu@163.com ; 15615748748@163.com ; ltj3987@163.com ; zhzhang@sdau.edu.cn ; zhxy@sdau.edu.cn ; lxz@sdau.edu.cn ; jhxnyx@sdau.edu.cn ; wangbc@ibcas.ac.cn ; linhj521@163.com ; dinghp@sdau.edu.cn ; pangt@sicau.edu.cn
作品OA属性	gold, Green Published
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28841
专题	中科院光生物学重点实验室
作者单位	1.Sichuan Agr Univ, Maize Res Inst, Chengdu 611130, Peoples R China 2.Guangdong Acad Agr Sci, Crops Res Inst, Guangzhou 510640, Peoples R China 3.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China 4.Chinese Acad Sci, Inst Bot, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Li, Qigui,Nie, Shujun,Li, Gaoke,et al. Identification and Fine Mapping of the Recessive Gene BK-5, Which Affects Cell Wall Biosynthesis and Plant Brittleness in Maize[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(2).
APA	Li, Qigui.,Nie, Shujun.,Li, Gaoke.,Du, Jiyuan.,Ren, Ruchang.,...&Pan, Guangtang.(2022).Identification and Fine Mapping of the Recessive Gene BK-5, Which Affects Cell Wall Biosynthesis and Plant Brittleness in Maize.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(2).
MLA	Li, Qigui,et al."Identification and Fine Mapping of the Recessive Gene BK-5, Which Affects Cell Wall Biosynthesis and Plant Brittleness in Maize".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.2(2022).

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