Full-length transcriptome-referenced analysis reveals crucial roles of hormone and wounding during induction of aerial bulbils in lily

doi:10.1186/s12870-022-03801-8

	Full-length transcriptome-referenced analysis reveals crucial roles of hormone and wounding during induction of aerial bulbils in lily
	Li, Jingrui; Sun, Meiyu; Li, Hui; Ling, Zhengyi; Wang, Di; Zhang, Jinzheng; Shi, Lei
	2022
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	22 期号:1
摘要	Aerial bulbils are important vegetative reproductive organs in Lilium. They are often perpetually dormant in most Lilium species, and little is known about the induction of these vegetative structures. The world-famous Oriental hybrid lily cultivar 'Sorbonne', which blooms naturally devoid of aerial bulbils, is known for its lovely appearance and sweet fragrance. We found that decapitation stimulated the outgrowth of aerial bulbils at lower stems (LSs) and then application of low and high concentrations of IAA promoted aerial bulbils emergence around the wound at upper stems (USs) of 'Sorbonne'. However, the genetic basis of aerial bulbil induction is still unclear. Herein, 'Sorbonne' transcriptome has been sequenced for the first time using the combination of third-generation long-read and next-generation short-read technology. A total of 46,557 high-quality non-redundant full-length transcripts were generated. Transcriptomic profiling was performed on seven tissues and stems with treatments of decapitation and application of low and high concentrations of IAA, respectively. Functional annotation of 1918 DEGs within stem samples of different treatments showed that hormone signaling, sugar metabolism and wound-induced genes were crucial to bulbils outgrowth. The expression pattern of auxin-, shoot branching hormone-, plant defense hormone- and wound-inducing-related genes indicated their crucial roles in bulbil induction. Then we established five hormone- and wounding-regulated co-expression modules and identified some candidate transcriptional factors, such as MYB, bZIP, and bHLH, that may function in inducing bulbils. High connectivity was observed among hormone signaling genes, wound-induced genes, and some transcriptional factors, suggesting wound- and hormone-invoked signals exhibit extensive cross-talk and regulate bulbil initiation-associated genes via multilayered regulatory cascades. We propose that the induction of aerial bulbils at LSs after decapitation can be explained as the release of apical dominance. In contrast, the induction of aerial bulbils at the cut surface of USs after IAA application occurs via a process similar to callus formation. This study provides abundant candidate genes that will deepen our understanding of the regulation of bulbil outgrowth, paving the way for further molecular breeding of lily.
关键词	Bulbil induction Full-length transcriptome Oriental hybrid lily cultivar 'Sorbonne' Hormone- and wounding-regulated network
学科领域	Plant Sciences
DOI	10.1186/s12870-022-03801-8
收录类别	SCI
语种	英语
WOS关键词	AUXIN ; GENES ; ARABIDOPSIS ; EXPRESSION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000846215900001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	Chinese Academy of Sciences [KFZD-SW-313] ; National High Technology Research and Development Program of China [2011AA10020805]
作者邮箱	shilei_67@126.com
作品OA属性	Green Published, gold
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28843
专题	中科院北方资源植物重点实验室
作者单位	Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Xiangshan, Peoples R China; Chinese Acad Sci, Inst Bot, China Natl Bot Garden, Beijing 100093, Xiangshan, Peoples R China
推荐引用方式 GB/T 7714	Li, Jingrui,Sun, Meiyu,Li, Hui,et al. Full-length transcriptome-referenced analysis reveals crucial roles of hormone and wounding during induction of aerial bulbils in lily[J]. BMC PLANT BIOLOGY,2022,22(1).
APA	Li, Jingrui.,Sun, Meiyu.,Li, Hui.,Ling, Zhengyi.,Wang, Di.,...&Shi, Lei.(2022).Full-length transcriptome-referenced analysis reveals crucial roles of hormone and wounding during induction of aerial bulbils in lily.BMC PLANT BIOLOGY,22(1).
MLA	Li, Jingrui,et al."Full-length transcriptome-referenced analysis reveals crucial roles of hormone and wounding during induction of aerial bulbils in lily".BMC PLANT BIOLOGY 22.1(2022).

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