Label-free quantitative proteomics reveals the antibacterial mechanism of rosemary essential oil against Salmonella enterica serovar Typhimurium

doi:10.1016/j.indcrop.2022.115757

	Label-free quantitative proteomics reveals the antibacterial mechanism of rosemary essential oil against Salmonella enterica serovar Typhimurium
	Hao, Yuanpeng 1; Guo, Xiaoqi 1; Zhang, Wenying 1; Xia, Fei ; Yang, Evan 2; Li, Hui ; Bai, Hongtong ; Shi, Lei
	2022
发表期刊	INDUSTRIAL CROPS AND PRODUCTS
ISSN	0926-6690
卷号	189
摘要	Rosemary essential oil (REO) shows various biological functions and is widely used in the cosmetic and pharmaceutical industries. Salmonella enterica serovar Typhimurium infection treated by antibiotics leads to serious drug resistance and public health hazard. REO as an antibiotic alternative has become the focus of considerable research efforts. However, knowledge regarding the S. Typhimurium growth characteristics and proteomic responses to REO exposure remains unclear. Here, we evaluated the commonalities and differences in chemical compositions of REOs extracted from Salvia rosmarinus cultivars, 'Algarve' (AL), 'Dutch Mill' (DM), and 'Majorca Pink' (MP) using gas chromatography-mass spectrometry. The three REOs displayed distinct chemical composition profiles, and the DM-REO, predominantly composed of 1,8-cineole and alpha-pinene, had the highest antibacterial activity. Sublethal levels of DM-REO caused irreversible cell membrane damage, longer lag phases, decreased growth rates, and lower maximum optical densities of S. Typhimurium. The underlying molecular regulatory mechanisms of S. Typhimurium under sublethal DM-REO treatment were investigated using label-free quantitative proteomics. Four hundred thirty-six differentially expressed proteins mainly involved in metabolism, ABC transporters, two-component system, quorum sensing, bacterial chemotaxis, and flagellar assembly were identified. The phenotypic observation test showed that DM-REO significantly inhibited S. Typhimurium motility and quorum sensing. These results increase our understanding of the antibacterial mechanism of REO inhibition in S. Typhimurium.
关键词	Rosemary essential oil Salmonella enterica serovar Typhimurium Antimicrobial mechanism Growth parameters Proteomics
学科领域	Agricultural Engineering ; Agronomy
DOI	10.1016/j.indcrop.2022.115757
收录类别	SCI
语种	英语
WOS关键词	ROSMARINUS-OFFICINALIS ; ANTIOXIDANT PROPERTIES ; L.
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000906234400003
出版者	ELSEVIER
文献子类	Article
出版地	AMSTERDAM
EISSN	1872-633X
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences [XDA23080603] ; R&D Program of Sinno Cosmetics Co., Ltd [2019C079]
作者邮箱	shilei_67@126.com
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28846
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Sinno Cosmet Co Ltd, Zhongshan 528451, Peoples R China
推荐引用方式 GB/T 7714	Hao, Yuanpeng,Guo, Xiaoqi,Zhang, Wenying,et al. Label-free quantitative proteomics reveals the antibacterial mechanism of rosemary essential oil against Salmonella enterica serovar Typhimurium[J]. INDUSTRIAL CROPS AND PRODUCTS,2022,189.
APA	Hao, Yuanpeng.,Guo, Xiaoqi.,Zhang, Wenying.,Xia, Fei.,Yang, Evan.,...&Shi, Lei.(2022).Label-free quantitative proteomics reveals the antibacterial mechanism of rosemary essential oil against Salmonella enterica serovar Typhimurium.INDUSTRIAL CROPS AND PRODUCTS,189.
MLA	Hao, Yuanpeng,et al."Label-free quantitative proteomics reveals the antibacterial mechanism of rosemary essential oil against Salmonella enterica serovar Typhimurium".INDUSTRIAL CROPS AND PRODUCTS 189(2022).

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