Knowledge Management System Of Institute Of Botany,CAS
| Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells | |
| Kong, Junhua3,4; Garcia, Virginie; Zehraoui, Enric; Stammitti, Linda; Hilbert, Ghislaine; Renaud, Christel; Maury, Stephane1; Delaunay, Alain1; Cluzet, Stephanie2; Lecourieux, Fatma; Lecourieux, David; Teyssier, Emeline; Gallusci, Philippe | |
| 2022 | |
| 发表期刊 | GENES
 |
| 卷号 | 13期号:7 |
| 摘要 | Through its role in the regulation of gene expression, DNA methylation can participate in the control of specialized metabolite production. We have investigated the link between DNA methylation and anthocyanin accumulation in grapevine using the hypomethylating drug, zebularine and Gamay Teinturier cell suspensions. In this model, zebularine increased anthocyanin accumulation in the light, and induced its production in the dark. To unravel the underlying mechanisms, cell transcriptome, metabolic content, and DNA methylation were analyzed. The up-regulation of stress-related genes, as well as a decrease in cell viability, revealed that zebularine affected cell integrity. Concomitantly, the global DNA methylation level was only slightly decreased in the light and not modified in the dark. However, locus-specific analyses demonstrated a decrease in DNA methylation at a few selected loci, including a CACTA DNA transposon and a small region upstream from the UFGT gene, coding for the UDP glucose:flavonoid-3-O-glucosyltransferase, known to be critical for anthocyanin biosynthesis. Moreover, this decrease was correlated with an increase in UFGT expression and in anthocyanin content. In conclusion, our data suggest that UFGT expression could be regulated through DNA methylation in Gamay Teinturier, although the functional link between changes in DNA methylation and UFGT transcription still needs to be demonstrated. |
| 关键词 | DNA methylation zebularine anthocyanin |
| 学科领域 | Genetics & Heredity |
| DOI | 10.3390/genes13071256 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | GLUCOSE-FLAVONOID 3-O-GLUCOSYLTRANSFERASE ; TRANSCRIPTION FACTOR ; PROANTHOCYANIDIN BIOSYNTHESIS ; STILBENE BIOSYNTHESIS ; CYTOSINE METHYLATION ; SUSPENSION CULTURES ; METABOLIC PATHWAYS ; GAMMA-IRRADIATION ; SYNTHASE GENES ; PLANT STRESS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000833132100001 |
| 出版者 | MDPI |
| 文献子类 | Article |
| 出版地 | BASEL |
| EISSN | 2073-4425 |
| 资助机构 | China Scholarship Council |
| 作者邮箱 | kongjunhua@ibcas.ac.cn ; virginie.garcia@inrae.fr ; enric.zehraoui@inrae.fr ; linda.stammitti@inrae.fr ; ghislaine.hilbert@inrae.fr ; christel.renaud@inrae.fr ; stephane.maury@univ-orleans.fr ; alain.delaunay@univ-orleans.fr ; stephanie.cluzet@u-bordeaux.fr ; fatma.ouaked-lecourieux@inrae.fr ; david.lecourieux@inrae.fr ; emeline.teyssier@inrae.fr ; philippe.gallusci@inrae.fr |
| 作品OA属性 | Green Published, gold |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/28848 |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Univ Bordeaux, Bordeaux Sci Agro, INRAE, UMR Ecophysiol & Genom Fonct Vigne, 210 Chemin Leysotte, F-33140 Villenave Dornon, France 2.Univ Orleans, EA1207 USC1328 Lab Biol Ligneux & Grandes Culture, INRAe, F-45067 Orleans, France 3.Univ Bordeaux, Fac Sci Pharmaceut, Unite Rech Oenol, USC INRA 1366,ISVV,Equipe Mol Interet Biol GESVAB, F-33882 Villenave Dornon, France 4.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China 5.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Kong, Junhua,Garcia, Virginie,Zehraoui, Enric,et al. Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells[J]. GENES,2022,13(7). |
| APA | Kong, Junhua.,Garcia, Virginie.,Zehraoui, Enric.,Stammitti, Linda.,Hilbert, Ghislaine.,...&Gallusci, Philippe.(2022).Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells.GENES,13(7). |
| MLA | Kong, Junhua,et al."Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells".GENES 13.7(2022). |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Kong-2022-Zebularine（5519KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论