Overexpression of HLH4 Inhibits Cell Elongation and Anthocyanin Biosynthesis in Arabidopsis thaliana

doi:10.3390/cells11071087

	Overexpression of HLH4 Inhibits Cell Elongation and Anthocyanin Biosynthesis in Arabidopsis thaliana
	Hou, Quancan 1,2; Zhao, Wei ; Lu, Lu ; Wang, Linlin ; Zhang, Tianye ; Hu, Binbin 3; Yan, Tingwei ; Qi, Yuchen ; Zhang, Fan ; Chao, Nan 4; Bartels, Dorothea 2; Wan, Xiangyuan 1
	2022
发表期刊	CELLS
卷号	11 期号:7
摘要	In plants, many basic helix-loop-helix (bHLH) transcription factors are involved in controlling cell elongation. Three bHLH proteins, PACLOBTRAZOL RESISTANCE1 (PRE1), Cryptochrome Interacting Basic Helix-loop-helix 5 (CIB5), and Arabidopsis ILI1 binding bHLH1 (IBH1) form a triantagonistic system that antagonistically regulates cell elongation in a competitive manner. In this study, we identified a new player, HLH4, related to IBH1, that negatively regulates cell elongation in Arabidopsis thaliana. Overexpression of HLH4 causes dwarf and dark green phenotypes and results in the downregulation of many key regulatory and enzymatic genes that participate in the anthocyanin biosynthetic pathway. HLH4 interacts with CIB5 and PRE1. By interacting with CIB5, HLH4 interferes with the activity of CIB5, and thus inhibiting the transcription of cell elongation-related genes regulated by CIB5, including EXPANSINS8 and 11 (EXP8 and EXP11) and indole-3-acetic acid 7 and 17 (IAA7 and IAA17). The interference of HLH4 on CIB5 is counteracted by PRE1, in which these bHLH proteins form a new tri-antagonistic system.
关键词	anthocyanin biosynthesis cell elongation bHLH transcription factor HLH4 HLH bHLH triantagonistic system
学科领域	Cell Biology
DOI	10.3390/cells11071087
收录类别	SCI
语种	英语
WOS关键词	LOOP-HELIX PROTEINS ; TRANSCRIPTION FACTORS ; SIGNAL-TRANSDUCTION ; GENE-EXPRESSION ; SHADE AVOIDANCE ; PHYTOCHROME-B ; GENOME-WIDE ; RESPONSES ; FAMILY ; GIBBERELLIN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000782217300001
出版者	MDPI
文献子类	Article
出版地	BASEL
EISSN	2073-4409
资助机构	National Key Research and Development Program of China [2021YFD1200700] ; Beijing Science and Technology Plan Program [Z201100006820114] ; Fundamental Research Funds for the Central Universities of China [06500136] ; China Scholarship Council [2010630118]
作者邮箱	houquancan@ustb.edu.cn ; b20190396@xs.ustb.edu.cn ; g20198927@xs.ustb.edu.cn ; s20200896@xs.ustb.edu.cn ; s20200906@xs.ustb.edu.cn ; hubinbin@ibcas.ac.cn ; b20190395@xs.ustb.edu.cn ; m202110892@xs.ustb.edu.cn ; b20200418@xs.ustb.edu.cn ; 201900000066@just.edu.cn ; dbartels@uni-bonn.de ; wanxiangyuan@ustb.edu.cn
作品OA属性	gold, Green Published
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28861
专题	中科院植物分子生理学重点实验室
作者单位	1.Univ Sci & Technol Beijing USTB, Res Ctr Biol & Agr, Shunde Grad Sch, Zhongzhi Int Inst Agr Biosci, Beijing 100024, Peoples R China 2.Beijing Solidwill Sci Tech Co Ltd, Beijing Engn Lab Main Crop Biotech Breeding, Beijing Int Sci & Technol Cooperat Base Biotech B, Beijing 100192, Peoples R China 3.Univ Bonn, Inst Mol Physiol & Biotechnol Plants, Kirschallee 1, D-53315 Bonn, Germany 4.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 5.Jiangsu Univ Sci & Technol, Coll Biotechnol, Zhenjiang 212018, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Hou, Quancan,Zhao, Wei,Lu, Lu,et al. Overexpression of HLH4 Inhibits Cell Elongation and Anthocyanin Biosynthesis in Arabidopsis thaliana[J]. CELLS,2022,11(7).
APA	Hou, Quancan.,Zhao, Wei.,Lu, Lu.,Wang, Linlin.,Zhang, Tianye.,...&Wan, Xiangyuan.(2022).Overexpression of HLH4 Inhibits Cell Elongation and Anthocyanin Biosynthesis in Arabidopsis thaliana.CELLS,11(7).
MLA	Hou, Quancan,et al."Overexpression of HLH4 Inhibits Cell Elongation and Anthocyanin Biosynthesis in Arabidopsis thaliana".CELLS 11.7(2022).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Hou-2022-Overexpress（5182KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文