Unraveling the Biosynthesis of Carvacrol in Different Tissues of Origanum vulgare

doi:10.3390/ijms232113231

	Unraveling the Biosynthesis of Carvacrol in Different Tissues of Origanum vulgare
	Hao, Yuanpeng 1,2; Guo, Xiaoqi 1,2; Yang, Rui 1,2; Yan, Yihao 1,2; Sun, Meiyu 1; Li, Hui 1; Bai, Hongtong 1; Cui, Hongxia 1; Li, Jingyi 3; Shi, Lei1
	2022
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	23 期号:21
摘要	Origanum vulgare, belonging to the Lamiaceae family, is a principal culinary herb used worldwide which possesses great antioxidant and antibacterial properties corresponding to various volatile organic components (VOCs). However, the metabolite profiles and underlying biosynthesis mechanisms of elaborate tissues (stems, leaves, bracts, sepals, petals) of Origanum vulgare have seldom been reported. Here, solid-phase microextraction-gas chromatography/mass spectrometry results showed that Origanum vulgare 'Hot and Spicy' (O. vulgare 'HS') was extremely rich in carvacrol and had the tissue dependence characteristic. Moreover, a full-length transcriptome analysis revealed carvacrol biosynthesis and its tissue-specific expression patterns of 'upstream' MVA/MEP pathway genes and 'downstream' modifier genes of TPSs, CYPs, and SDRs. Furthermore, the systems biology method of modular organization analysis was applied to cluster 16,341 differently expressed genes into nine modules and to identify significant carvacrol- and peltate glandular trichome-correlated modules. In terms of these positive and negative modules, weighted gene co-expression network analysis results showed that carvacrol biosynthetic pathway genes are highly co-expressed with TF genes, such as ZIPs and bHLHs, indicating their involvement in regulating the biosynthesis of carvacrol. Our findings shed light on the tissue specificity of VOC accumulation in O. vulgare 'HS' and identified key candidate genes for carvacrol biosynthesis, which would allow metabolic engineering and breeding of Origanum cultivars.
关键词	Origanum vulgare carvacrol tissue dependence SMRT WGCNA
学科领域	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
DOI	10.3390/ijms232113231
收录类别	SCI
语种	英语
WOS关键词	GERANYL DIPHOSPHATE SYNTHASE ; TRANSCRIPTION FACTOR ; GLANDULAR TRICHOMES ; TERPENE SYNTHASES ; ESSENTIAL OILS ; EXPRESSION ; ORGANIZATION ; SUPPRESSES ; METABOLISM ; DIVERSITY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000883971700001
出版者	MDPI
文献子类	Article
出版地	BASEL
EISSN	1422-0067
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences [XDA26040306] ; NationalWild Plant Germplasm Resource Center
作者邮箱	lijingyi@imma.ac.cn ; shilei@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28863
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.China Natl Bot Garden, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Med Sci & Peking Union Med Coll, Inst Mat Med, Beijing 100050, Peoples R China
推荐引用方式 GB/T 7714	Hao, Yuanpeng,Guo, Xiaoqi,Yang, Rui,et al. Unraveling the Biosynthesis of Carvacrol in Different Tissues of Origanum vulgare[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(21).
APA	Hao, Yuanpeng.,Guo, Xiaoqi.,Yang, Rui.,Yan, Yihao.,Sun, Meiyu.,...&Shi, Lei.(2022).Unraveling the Biosynthesis of Carvacrol in Different Tissues of Origanum vulgare.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(21).
MLA	Hao, Yuanpeng,et al."Unraveling the Biosynthesis of Carvacrol in Different Tissues of Origanum vulgare".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.21(2022).

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