Heat production and volatile biosynthesis are linked via alternative respiration in Magnolia denudata during floral thermogenesis

doi:10.3389/fpls.2022.955665

	Heat production and volatile biosynthesis are linked via alternative respiration in Magnolia denudata during floral thermogenesis
	Wang, Ruohan 1; Chen, Ling 1; Jia, Yaping 1; Liu, Liya 2; Sun, Liwei 1; Liu, Yujun 1; Li, Yun 1
	2022
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	13
摘要	Floral thermogenesis is coupled with odor emission in known thermogenic plants. It is widely accepted that elevation in floral temperature can help release of volatile organic compounds (VOCs). However, no information is available about whether floral thermogenesis is associated with VOC biosynthesis. Here, we used RNA-Sequencing (RNA-Seq) to draw a gene expression atlas of floral thermogenesis in Magnolia denudata and captured an upregulation of Alternative Oxidase (AOX) during floral thermogenesis. Western blot analyses also suggested upregulation of AOX during floral thermogenesis. Moreover, oxygen consumption analyses revealed increased activity of the AOX respiration pathway during floral thermogenesis. Using HPLC analyses, we further found that increased AOX respiration substantially promoted production of citric acid by 1.35 folds, which provided fundamental metabolite skeletons for biosynthesis of VOCs. RNA-Seq also showed upregulation of genes regulating lignin catabolism, which was in agreement with in situ Raman chemical imaging of lignin. Taken together, our results suggest the central role of AOX by coupling heat production and VOC biosynthesis in floral thermogenesis of M. denudata.
关键词	floral thermogenesis high-throughput sequencing alternative respiration cell wall volatile organic compounds
学科领域	Plant Sciences
DOI	10.3389/fpls.2022.955665
收录类别	SCI
语种	英语
WOS关键词	SKUNK CABBAGE ; UNCOUPLING-PROTEIN ; TEMPERATURE REGULATION ; LABEL-FREE ; MITOCHONDRIAL ; POLLINATION ; PATHWAY ; EXPRESSION ; OXIDASE ; SPADIX
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000876754000001
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
资助机构	Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; [2021ZY63] ; [31770201]
作者邮箱	wangrh@bjfu.edu.cn
作品OA属性	gold, Green Published
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28881
专题	中科院植物分子生理学重点实验室
作者单位	1.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Natl Engn Lab Tree Breeding, Beijing, Peoples R China 2.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Key Lab Genet & Breeding Forest Trees & Ornamental, Minist Educ, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ruohan,Chen, Ling,Jia, Yaping,et al. Heat production and volatile biosynthesis are linked via alternative respiration in Magnolia denudata during floral thermogenesis[J]. FRONTIERS IN PLANT SCIENCE,2022,13.
APA	Wang, Ruohan.,Chen, Ling.,Jia, Yaping.,Liu, Liya.,Sun, Liwei.,...&Li, Yun.(2022).Heat production and volatile biosynthesis are linked via alternative respiration in Magnolia denudata during floral thermogenesis.FRONTIERS IN PLANT SCIENCE,13.
MLA	Wang, Ruohan,et al."Heat production and volatile biosynthesis are linked via alternative respiration in Magnolia denudata during floral thermogenesis".FRONTIERS IN PLANT SCIENCE 13(2022).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
fpls-13-955665.pdf（4987KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文