Microtubules promote the non-cell autonomous action of microRNAs by inhibiting their cytoplasmic loading onto ARGONAUTE1 in Arabidopsis

doi:10.1016/j.devcel.2022.03.015

	Microtubules promote the non-cell autonomous action of microRNAs by inhibiting their cytoplasmic loading onto ARGONAUTE1 in Arabidopsis
	Fan, Lusheng ; Zhang, Cui1 ; Gao, Bin ; Zhang, Yong ; Stewart, Ethan 2; Jez, Jakub 2; Nakajima, Keiji 3; Chen, Xuemei
	2022
发表期刊	DEVELOPMENTAL CELL
ISSN	1534-5807
卷号	57 期号:8 页码:995-1008
摘要	Mobile microRNAs (miRNAs) serve as local and long-distance signals in the developmental patterning and stress responses in plants. However, mechanisms governing the non-cell autonomous activities of miRNAs remain elusive. Here, we show that mutations that disrupt microtubule dynamics are specifically defective for the non-cell autonomous actions of mobile miRNAs, including miR165/6 that is produced in the endodermis and moves to the vasculature to pattern xylem cell fates in Arabidopsis roots. We show that KTN1, a subunit of a microtubule-severing enzyme, is required in source cells to inhibit the loading of miR165/6 into ARGONUATE1 (AGO1), which is cell autonomous, to enable the miRNA to exit the cell. Microtubule disruption enhances the association of miR165/6 with AGO1 in the cytoplasm. These findings suggest that although cell-autonomous miRNAs load onto AGO1 in the nucleus, the cytoplasmic AGO1 loading of mobile miRNAs is a key step regulated by microtubules to promote the range of miRNA cell-to-cell movement.
学科领域	Cell Biology ; Developmental Biology
DOI	10.1016/j.devcel.2022.03.015
收录类别	SCI
语种	英语
WOS关键词	SMALL RNAS ; MOBILE ; MOVEMENT ; DIFFERENTIATION ; SPECIFICATION ; BIOSYNTHESIS ; LOCALIZATION ; BIOGENESIS ; NETWORK ; MIRNAS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000832106600005
出版者	CELL PRESS
文献子类	Article
出版地	CAMBRIDGE
EISSN	1878-1551
资助机构	National Institutes of Health [GM129373]
作者邮箱	xuemei.chen@ucr.edu
作品OA属性	Green Submitted, hybrid, Green Accepted
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28889
专题	中科院植物分子生理学重点实验室
作者单位	1.Univ Calif Riverside, Dept Bot & Plant Sci, Inst Integrat Genome Biol, Riverside, CA 92521 USA 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Vienna Bioctr Core Facil VBCF, Vienna, Austria 4.Nara Inst Sci & Technol, Grad Sch Sci & Technol, 8916-5 Takayama, Nara 6300192, Japan
推荐引用方式 GB/T 7714	Fan, Lusheng,Zhang, Cui,Gao, Bin,et al. Microtubules promote the non-cell autonomous action of microRNAs by inhibiting their cytoplasmic loading onto ARGONAUTE1 in Arabidopsis[J]. DEVELOPMENTAL CELL,2022,57(8):995-1008.
APA	Fan, Lusheng.,Zhang, Cui.,Gao, Bin.,Zhang, Yong.,Stewart, Ethan.,...&Chen, Xuemei.(2022).Microtubules promote the non-cell autonomous action of microRNAs by inhibiting their cytoplasmic loading onto ARGONAUTE1 in Arabidopsis.DEVELOPMENTAL CELL,57(8),995-1008.
MLA	Fan, Lusheng,et al."Microtubules promote the non-cell autonomous action of microRNAs by inhibiting their cytoplasmic loading onto ARGONAUTE1 in Arabidopsis".DEVELOPMENTAL CELL 57.8(2022):995-1008.

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