Knowledge Management System Of Institute Of Botany,CAS
| Excited-state intermediates in a designer protein encoding a phototrigger caught by an X-ray free-electron laser | |
Liu, Xiaohong; Liu, Pengcheng; Li, Hongjie; Xu, Zhen; Jia, Lu; Xia, Yan; Yu, Minling; Tang, Wenqin; Zhu, Xiaolei; Chen, Chao; Zhang, Yuanlin3,4; Nango, Eriko4,5; Tanaka, Rie4,5; Luo, Fangjia; Kato, Koji; Nakajima, Yoshiki; Kishi, Shunpei6; Yu, Huaxin; Matsubara, Naoki; Owada, Shigeki4,7; Tono, Kensuke4,7; Iwata, So4,5; Yu, Long-Jiang ; Shen, Jian-Ren; Wang, Jiangyun
| |
| 2022 | |
| 发表期刊 | NATURE CHEMISTRY
 |
| ISSN | 1755-4330 |
| 卷号 | 14期号:9页码:1054+ |
| 摘要 | One of the primary objectives in chemistry research is to observe atomic motions during reactions in real time. Although X-ray free-electron lasers (XFELs) have facilitated the capture of reaction intermediates using time-resolved serial femtosecond crystallography (TR-SFX), only a few natural photoactive proteins have been investigated using this method, mostly due to the lack of suitable phototriggers. Here we report the genetic encoding of a xanthone amino acid (FXO), as an efficient phototrigger, into a rationally designed human liver fatty-acid binding protein mutant (termed XOM), which undergoes photo-induced C-H bond transformation with high selectivity and quantum efficiency. We solved the structures of XOM before and 10-300 ns after flash illumination, at 1.55-1.70 angstrom resolutions, and captured the elusive excited-state intermediates responsible for precise C-H bond activation. We expect that most redox enzymes can now be investigated by TR-SFX, using our method, to reveal reaction intermediates key for their efficiency and selectivity. |
| 学科领域 | Chemistry, Multidisciplinary |
| DOI | 10.1038/s41557-022-00992-3 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | EXCIPLEX FORMATION ; STRUCTURAL-CHANGES ; CRYSTALLOGRAPHY ; MYOGLOBIN ; PATHWAYS ; EXCIMERS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000826794700003 |
| 出版者 | NATURE PORTFOLIO |
| 文献子类 | Article |
| 出版地 | BERLIN |
| EISSN | 1755-4349 |
| 资助机构 | National Science Foundation of China [22193023, 22121003, 91953202, 21837005] ; National Key Research and Development Program of China [2017YFA0503704, 2017YFA0503700, 2019YFA0904101] ; CAS Project for Young Scientists in Basic Research [YSBR-015] ; Sanming Project of Medicine in Shenzhen [SZSM201811092] ; Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED [JP20am0101070] ; JSPS KAKENHI [JP18H02394] |
| 作者邮箱 | longer@ibcas.ac.cn ; shen@cc.okayama-u.ac.jp ; jwang@ibp.ac.cn |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/28890 |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Biophys, Lab RNA Biol, Beijing, Peoples R China 2.Okayama Univ, Res Inst Interdisciplinary Sci, Okayama, Japan 3.Okayama Univ, Grad Sch Nat Sci & Technol, Okayama, Japan 4.Tohoku Univ, Inst Multidisciplinary Res Adv Mat, Sendai, Miyagi, Japan 5.RIKEN SPring 8 Ctr, Kobe, Hyogo, Japan 6.Kyoto Univ, Grad Sch Med, Dept Cell Biol, Kyoto, Japan 7.Univ Hyogo, Grad Sch Life Sci, Dept Picobiol, Kobe, Hyogo, Japan 8.Japan Synchrotron Radiat Res Inst, Kobe, Hyogo, Japan 9.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xiaohong,Liu, Pengcheng,Li, Hongjie,et al. Excited-state intermediates in a designer protein encoding a phototrigger caught by an X-ray free-electron laser[J]. NATURE CHEMISTRY,2022,14(9):1054+. |
| APA | Liu, Xiaohong.,Liu, Pengcheng.,Li, Hongjie.,Xu, Zhen.,Jia, Lu.,...&Wang, Jiangyun.(2022).Excited-state intermediates in a designer protein encoding a phototrigger caught by an X-ray free-electron laser.NATURE CHEMISTRY,14(9),1054+. |
| MLA | Liu, Xiaohong,et al."Excited-state intermediates in a designer protein encoding a phototrigger caught by an X-ray free-electron laser".NATURE CHEMISTRY 14.9(2022):1054+. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Excited_state_interm（1994KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论