The transcription factor LaMYC4 from lavender regulates volatile Terpenoid biosynthesis

doi:10.1186/s12870-022-03660-3

	The transcription factor LaMYC4 from lavender regulates volatile Terpenoid biosynthesis
	Dong, Yanmei 1; Zhang, Wenying 1; Li, Jingrui ; Wang, Di ; Bai, Hongtong ; Li, Hui ; Shi, Lei
	2022
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	22 期号:1
摘要	Background The basic helix-loop-helix (bHLH) transcription factors (TFs), as one of the largest families of TFs, are essential regulators of plant terpenoid biosynthesis and response to stresses. Lavender has more than 75 volatile terpenoids, yet few TFs have been identified to be involved in the terpenoid biosynthesis. Results Based on RNA-Seq, reverse transcription-quantitative polymerase chain reaction, and transgenic technology, this study characterized the stress-responsive transcription factor LaMYC4 regulates terpenoid biosynthesis. Methyl jasmonate (MeJA) treatment increased volatile terpenoid emission, and the differentially expressed gene LaMYC4 was isolated. LaMYC4 expression level was higher in leaf than in other tissues. The expression of LaMYC4 decreased during flower development. The promoter of LaMYC4 contained hormone and stress-responsive regulatory elements and was responsive to various treatments, including UV, MeJA treatment, drought, low temperature, Pseudomonas syringae infection, and NaCl treatment. LaMYC4 overexpression increased the levels of sesquiterpenoids, including caryophyllenes, in Arabidopsis and tobacco plants. Furthermore, the expression of crucial node genes involved in terpenoid biosynthesis and glandular trichome number and size increased in transgenic tobacco. Conclusions We have shown that the stress-responsive MYC TF LaMYC4 from 'Jingxun 2' lavender regulates volatile terpenoid synthesis. This study is the first to describe the cloning of LaMYC4, and the results help understand the role of LaMYC4 in terpenoid biosynthesis.
关键词	Lavandula angustifolia Molecular cloning bHLH transcription factors Stress-responsive Terpenoid biosynthesis
学科领域	Plant Sciences
DOI	10.1186/s12870-022-03660-3
收录类别	SCI
语种	英语
WOS关键词	ARTEMISININ BIOSYNTHESIS ; SIGNALING PATHWAY ; DROUGHT TOLERANCE ; GENES ; BHLH ; STRESS ; EXPRESSION ; INCREASES ; INTERACTS ; PROTEINS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000810684100001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	[DC3000]
作者邮箱	lihuibjfu@126.com ; shilei_67@126.com
作品OA属性	gold, Green Published
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28900
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources & Beijing Bot Garden, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100015, Peoples R China
推荐引用方式 GB/T 7714	Dong, Yanmei,Zhang, Wenying,Li, Jingrui,et al. The transcription factor LaMYC4 from lavender regulates volatile Terpenoid biosynthesis[J]. BMC PLANT BIOLOGY,2022,22(1).
APA	Dong, Yanmei.,Zhang, Wenying.,Li, Jingrui.,Wang, Di.,Bai, Hongtong.,...&Shi, Lei.(2022).The transcription factor LaMYC4 from lavender regulates volatile Terpenoid biosynthesis.BMC PLANT BIOLOGY,22(1).
MLA	Dong, Yanmei,et al."The transcription factor LaMYC4 from lavender regulates volatile Terpenoid biosynthesis".BMC PLANT BIOLOGY 22.1(2022).

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