Condensation of SEUSS promotes hyperosmotic stress tolerance in Arabidopsis

doi:10.1038/s41589-022-01196-z

IB-CAS > 中科院光生物学重点实验室

	Condensation of SEUSS promotes hyperosmotic stress tolerance in Arabidopsis
	Wang, Boyu ; Zhang, Honghong ; Huai, Junling 1; Peng, Fangyu ; Wu, Jie ; Lin, Rongcheng1 ; Fang, Xiaofeng
	2022
发表期刊	NATURE CHEMICAL BIOLOGY
ISSN	1552-4450
卷号	18 期号:12
摘要	Osmotic stress imposed by drought and high salinity inhibits plant growth and crop yield. However, our current knowledge on the mechanism by which plants sense osmotic stress is still limited. Here, we identify the transcriptional regulator SEUSS (SEU) as a key player in hyperosmotic stress response in Arabidopsis. SEU rapidly coalesces into liquid-like nuclear condensates when extracellular osmolarity increases. The intrinsically disordered region 1 (IDR1) of SEU is responsible for its condensation. IDR1 undergoes conformational changes to adopt more compact states after an increase in molecular crowding both in vitro and in cells, and two predicted alpha-helical peptides are required. SEU condensation is indispensable for osmotic stress tolerance, and loss of SEU dramatically compromises the expression of stress tolerance genes. Our work uncovers a critical role of biomolecular condensates in cellular stress perception and response and expands our understanding of the osmotic stress pathway.
学科领域	Biochemistry & Molecular Biology
DOI	10.1038/s41589-022-01196-z
收录类别	SCI
语种	英语
WOS关键词	REGULATORY PROTEINS ; PHASE-SEPARATION ; GENE-EXPRESSION ; DYNAMICS ; FAMILY ; LEUNIG ; VOLUME
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000883236000001
出版者	NATURE PORTFOLIO
文献子类	Article
出版地	BERLIN
EISSN	1552-4469
资助机构	National Natural Science Foundation of China [32161133001, 31800235, 32030009] ; Beijing Natural Science Foundation [JQ21020] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDB27030205]
作者邮箱	rclin@ibcas.ac.cn ; xffang@tsinghua.edu.cn
引用统计	被引频次：60[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28912
专题	中科院光生物学重点实验室
作者单位	1.Tsinghua Univ, Ctr Plant Biol, Sch Life Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Boyu,Zhang, Honghong,Huai, Junling,et al. Condensation of SEUSS promotes hyperosmotic stress tolerance in Arabidopsis[J]. NATURE CHEMICAL BIOLOGY,2022,18(12).
APA	Wang, Boyu.,Zhang, Honghong.,Huai, Junling.,Peng, Fangyu.,Wu, Jie.,...&Fang, Xiaofeng.(2022).Condensation of SEUSS promotes hyperosmotic stress tolerance in Arabidopsis.NATURE CHEMICAL BIOLOGY,18(12).
MLA	Wang, Boyu,et al."Condensation of SEUSS promotes hyperosmotic stress tolerance in Arabidopsis".NATURE CHEMICAL BIOLOGY 18.12(2022).

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