2,3-Butanedione suppresses gray mold of postharvest fruit by activating the autophagy of Botrytis cinerea

doi:10.1016/j.postharvbio.2022.112057

	2,3-Butanedione suppresses gray mold of postharvest fruit by activating the autophagy of Botrytis cinerea
	Li, Guangjin 1; Chen, Yong 1; Zhang, Zhanquan 1; Li, Boqiang 1; Chen, Tong 1; Tian, Shiping1
	2022
发表期刊	POSTHARVEST BIOLOGY AND TECHNOLOGY
ISSN	0925-5214
卷号	193
摘要	The volatile organic compounds produced by plants and microorganisms have been widely used for postharvest disease control due to their high antifungal capacity. In this research, a volatile product of citrate metabolism, 2,3-butanedione, was assayed against gray mold caused by Botrytis cinerea on postharvest fruit. 2,3-Butanedione treatment exhibited a distinct inhibitory effect on colony growth, conidial germination rate and germ tube length of B. cinerea in vitro and efficiently mitigated the disease prevalence of inoculated fruit under both artificial and natural inoculation conditions. Transcriptome analysis displayed that 2,3-butanedione treatment led to down-regulated expression of genes associated with arginine biosynthesis, ribosome biogenesis and several pathogenicity-related genes, including Bcpg1-3, BcNEP1, Bcboa2 and Bmpl. Furthermore, treatment led to increased genes expression related to proteolysis, peroxisome and autophagy. Among them, the autophagy pathway was most prominent, and key genes in this pathway (BcATGs and BcVPS45) were activated by 2,3-butanedione treatment. Moreover, numerous autophagic vacuoles containing cytoplasmic components were observed in the 2,3-butanedione-treated fungal cells. These results suggest that 2,3-butanedione inhibits gray mold on postharvest fruit by not only interfering the gene expression of fungal growth and pathogenicity in B. cinerea, but also inducing autophagic activity. Collectively, these results provide a theoretical basis for elucidating the underlying antifungal mechanism of 2,3-butanedione against B. cinerea.
关键词	2,3-Butanedione Botrytis cinerea Antifungal mechanism Transcriptome analysis Autophagy
学科领域	Agronomy ; Food Science & Technology ; Horticulture
DOI	10.1016/j.postharvbio.2022.112057
收录类别	SCI
语种	英语
WOS关键词	ARGININE-BIOSYNTHESIS ; GROWTH ; PATHOGENICITY ; HEALTH
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000888205500005
出版者	ELSEVIER
文献子类	Article
出版地	AMSTERDAM
EISSN	1873-2356
资助机构	National Natural Science Foundation of China [31930086, 32002099, 32072637, 31972475]
作者邮箱	tsp@ibcas.ac.cn
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28952
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Guangjin,Chen, Yong,Zhang, Zhanquan,et al. 2,3-Butanedione suppresses gray mold of postharvest fruit by activating the autophagy of Botrytis cinerea[J]. POSTHARVEST BIOLOGY AND TECHNOLOGY,2022,193.
APA	Li, Guangjin,Chen, Yong,Zhang, Zhanquan,Li, Boqiang,Chen, Tong,&Tian, Shiping.(2022).2,3-Butanedione suppresses gray mold of postharvest fruit by activating the autophagy of Botrytis cinerea.POSTHARVEST BIOLOGY AND TECHNOLOGY,193.
MLA	Li, Guangjin,et al."2,3-Butanedione suppresses gray mold of postharvest fruit by activating the autophagy of Botrytis cinerea".POSTHARVEST BIOLOGY AND TECHNOLOGY 193(2022).

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