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| A Ca2+-binding motif underlies the unusual properties of certain photosynthetic bacterial core light-harvesting complexes | |
Tani, Kazutoshi; Kobayashi, Kazumi1; Hosogi, Naoki1; Ji, Xuan-Cheng; Nagashima, Sakiko; Nagashima, Kenji V. P.; Izumida, Airi4; Inoue, Kazuhito3,4; Tsukatani, Yusuke5; Kanno, Ryo; Hall, Malgorzata; Yu, Long-Jiang ; Ishikawa, Isamu1; Okura, Yoshihiro1; Madigan, Michael T.; Mizoguchii, Akira; Humbel, Bruno M.; Kimura, Yukihiro9; Wang-Otomo, Zheng-Yu
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| 2022 | |
| 发表期刊 | JOURNAL OF BIOLOGICAL CHEMISTRY
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| 卷号 | 298期号:6 |
| 摘要 | The mildly thermophilic purple phototrophic bacterium Allochromatium tepidum provides a unique model for investigating various intermediate phenotypes observed between those of thermophilic and mesophilic counterparts. The core light-harvesting (LH1) complex from A. tepidum exhibits an absorption maximum at 890 nm and mildly enhanced thermostability, both of which are Ca2+-dependent. However, it is unknown what structural determinants might contribute to these properties. Here, we present a cryo-EM structure of the reaction center-associated LH1 complex at 2.81 angstrom resolution, in which we identify multiple pigment-binding alpha- and beta-polypeptides within an LH1 ring. Of the 16 alpha-polypeptides, we show that six (alpha 1) bind Ca2+ along with beta 1- or beta 3-polypeptides to form the Ca-2-binding sites. This structure differs from that of fully Ca2+-bound LH1 from Thermochromatium tepidum, enabling determination of the minimum structural requirements for Ca2+-binding. We also identified three amino acids (Trp44, Asp47, and le49) in the C-terminal region of the A. tepidum alpha 1-polypeptide that ligate each Ca ion, forming a Ca2+-binding WxxDxl motif that is conserved in all Ca2+ bound LH1 alpha-polypeptides from other species with reported structures. The partial Ca-2-bound structure further explains the unusual phenotypic properties observed for this bacterium in terms of its Ca2+-requirements for thermostability, spectroscopy, and phototrophic growth, and supports the hypothesis that A. tepidum may represent a transitional species between mesophilic and thermophilic purple sulfur bacteria. The characteristic arrangement of multiple appolypeptides also suggests a mechanism of molecular recognition in the expression and/or assembly of the LH1 complex that could be regulated through interactions with reaction center subunits. |
| 学科领域 | Biochemistry & Molecular Biology |
| DOI | 10.1016/j.jbc.2022.101967 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | Q(Y) RED-SHIFT ; LH1-RC COMPLEX ; THERMOCHROMATIUM-TEPIDUM ; CALCIUM-IONS ; BACTERIOCHLOROPHYLL ; SYSTEM ; THERMOSTABILITY |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000994013900001 |
| 出版者 | ELSEVIER |
| 文献子类 | Article |
| 出版地 | AMSTERDAM |
| EISSN | 1083-351X |
| 资助机构 | National Key R&D Program of China [2019YFA0904600] |
| 作者邮箱 | ktani@doc.medic.mie-u.acjp ; ykimura@people.kobe-u.ac.jp ; wang@m.ibaraki.acjp |
| 作品OA属性 | Green Published, gold |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/28967 |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Mie Univ, Grad Sch Med, Tsu, Mie, Japan 2.JEOL Ltd, EM Business Unit, 3-1-2 Musashino, Akishima, Tokyo, Japan 3.Ibaraki Univ, Fac Sci, Mito, Ibaraki, Japan 4.Kanagawa Univ, Res Inst Integrated Sci, Fac Sci, Hiratsuka, Kanagawa, Japan 5.Kanagawa Univ, Dept Biol Sci, Fac Sci, Hiratsuka, Kanagawa, Japan 6.Japan Agcy Marine Earth Sci & Technol JAMSTEC, Inst Extra Cutting Edge Sci & Technol Avant Garde, Yokosuka, Kanagawa, Japan 7.Kanno, Ryo; Hall, Malgorzata; Humbel, Bruno M.] Okinawa Inst Sci & Technol Grad Univ OIST, Res Support Div, Imaging Sect, Okinawa, Japan 8.Chinese Acad Sci, Inst Bot, Photosynthesis Res Ctr, Key Lab Photobiol, Beijing, Peoples R China 9.Madigan, Michael T.] Southern Illinois Univ, Sch Biol Sci, Dept Microbiol, Carbondale, IL USA 10.Kobe Univ, Grad Sch Agr, Dept Agrobiosci, Kobe, Hyogo, Japan |
| 推荐引用方式 GB/T 7714 | Tani, Kazutoshi,Kobayashi, Kazumi,Hosogi, Naoki,et al. A Ca2+-binding motif underlies the unusual properties of certain photosynthetic bacterial core light-harvesting complexes[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2022,298(6). |
| APA | Tani, Kazutoshi.,Kobayashi, Kazumi.,Hosogi, Naoki.,Ji, Xuan-Cheng.,Nagashima, Sakiko.,...&Wang-Otomo, Zheng-Yu.(2022).A Ca2+-binding motif underlies the unusual properties of certain photosynthetic bacterial core light-harvesting complexes.JOURNAL OF BIOLOGICAL CHEMISTRY,298(6). |
| MLA | Tani, Kazutoshi,et al."A Ca2+-binding motif underlies the unusual properties of certain photosynthetic bacterial core light-harvesting complexes".JOURNAL OF BIOLOGICAL CHEMISTRY 298.6(2022). |
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