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New insights on the analysis of phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') revealed by GC-MS/MS | |
Sun, Shanshan1; Wang, Shujuan2; Li, Shanshan1; Wei, Liping2; Wang, Xiaohan1; Wang, Yizhou1; Li, Bing1; Hu, Yang3; Wang, Liangsheng1 | |
2022 | |
发表期刊 | ANALYTICA CHIMICA ACTA |
ISSN | 0003-2670 |
卷号 | 1212 |
摘要 | Phytosterols exhibit a wide range of pharmacological activities and have no side-effects, which have attracted more and more attention. In this study, two pre-treatment methods (alkaline hydrolysis and acid-alkali hydrolysis) were performed in parallel for the first time to investigate the effects on phytosterol analysis. The aim is to develop an analytical method for phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') by gas chromatography-mass spectrometry. The result indicated that the contents of phytosterols in pollen (3390.02 mg/100 g DW) and anther wall (929.70 mg/100 g DW) of P. ostii 'Fengdan' were relatively high. Meanwhile, eleven phytosterols were identified, among which five phytosterols were first identified in tree peony pollen. Moreover, the effects of wall breaking and different developmental stages on phytosterol contents of pollen and anther wall of P. ostii 'Fengdan' were also discussed. The result showed that wall breaking was beneficial to sterol detection of pollen, but not necessary for anther wall. For the best harvest time, the stage of S2 had the highest content of phytosterols. In conclusion, this study successfully establishes an analytical method for phytosterols by gas chromatography-mass spectrometry, and lays a foundation for the development and utilization of pollen and anther wall of P. ostii 'Fengdan' in functional foods and pharmaceutical industry. |
关键词 | Phytosterol Paeonia ostii 'Fengdan' Pollen Anther wall Gas chromatography-mass spectrometry (GC-MS) |
学科领域 | Chemistry, Analytical |
DOI | 10.1016/j.aca.2022.339891 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | GAS-CHROMATOGRAPHY ; BEE POLLEN ; OILS ; PHYTOSTANOLS ; EXTRACTION ; FRACTION ; ESTERS ; FOODS ; SEEDS |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000830632800001 |
出版者 | ELSEVIER |
文献子类 | Article |
出版地 | AMSTERDAM |
EISSN | 1873-4324 |
资助机构 | National Natural Science Foundation of China [32030095] ; Science and Technology Project of Lu'an Group [2019C062] |
作者邮箱 | wanglsh@ibcas.ac.cn |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/28971 |
专题 | 中科院北方资源植物重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources & Beijing Bot Garden, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Shennong Zhihua Biotechnol Shanxi Co Ltd, Changzhi 046011, Peoples R China 4.Inner Mongolia Agr Univ, Coll Desert Management, Hohhot 010018, Peoples R China |
推荐引用方式 GB/T 7714 | Sun, Shanshan,Wang, Shujuan,Li, Shanshan,et al. New insights on the analysis of phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') revealed by GC-MS/MS[J]. ANALYTICA CHIMICA ACTA,2022,1212. |
APA | Sun, Shanshan.,Wang, Shujuan.,Li, Shanshan.,Wei, Liping.,Wang, Xiaohan.,...&Wang, Liangsheng.(2022).New insights on the analysis of phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') revealed by GC-MS/MS.ANALYTICA CHIMICA ACTA,1212. |
MLA | Sun, Shanshan,et al."New insights on the analysis of phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') revealed by GC-MS/MS".ANALYTICA CHIMICA ACTA 1212(2022). |
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