Highly active repeat-mediated recombination in the mitogenome of the holoparasitic plant Aeginetia indica

doi:10.3389/fpls.2022.988368

	Highly active repeat-mediated recombination in the mitogenome of the holoparasitic plant Aeginetia indica
	Zhong, Yan 1; Yu, Runxian 2,3; Chen, Jingfang 1; Liu, Ying 1; Zhou, Renchao 1
	2022
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	13
摘要	Mitogenomes of most flowering plants evolve slowly in sequence, but rapidly in structure. The rearrangements in structure are mainly caused by repeat-mediated recombination. However, patterns of repeat-mediated recombination vary substantially among plants, and to provide a comprehensive picture, characterization of repeat-mediated recombination should extend to more plant species, including parasitic plants with a distinct heterotrophic lifestyle. Here we assembled the mitogenome of the holoparasitic plant Aeginetia indica (Orobanchaceae) using Illumina sequencing reads. The mitogenome was assembled into a circular chromosome of 420,362 bp, 18,734 bp longer than that of another individual of A. indica which was assembled before as a linear molecule. Synteny analysis between the two mitogenomes revealed numerous rearrangements, unique regions of each individual and 0.2% sequence divergence in their syntenic regions. The A. indica mitogenome contains a gene content typical of flowering plants (33 protein-coding, 3 rRNA, and 17 tRNA genes). Repetitive sequences >30 bp in size totals 57,060 bp, representing 13.6% of the mitogenome. We examined recombination mediated by repeats >100 bp in size and found highly active recombination for all the repeats, including a very large repeat of similar to 16 kb. Recombination between these repeats can form much smaller subgenomic circular chromosomes, which may lead to rapid replication of mitochondrial DNA and thus be advantageous for A. indica with a parasitic lifestyle. In addition, unlike some other parasitic plants, A. indica shows no evidence for horizontal gene transfer of protein-coding genes in its mitogenome.
关键词	Aeginetia indica mitochondrial genome repeat-mediated recombination intraspecific variation HGT
学科领域	Plant Sciences
DOI	10.3389/fpls.2022.988368
收录类别	SCI
语种	英语
WOS关键词	MITOCHONDRIAL GENOMES ; EVOLUTION ; CHLOROPLAST ; RATES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000891395300001
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
资助机构	National Natural Science Foundation of China ; [31811530297]
作者邮箱	zhrench@mail.sysu.edu.cn
作品OA属性	gold, Green Published
引用统计
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28978
专题	系统与进化植物学国家重点实验室
作者单位	1.Sun Yat sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou, Peoples R China 2.Sun Yat sen Univ, Sch Life Sci, Guangdong Prov Key Lab Plant Resources, Guangzhou, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhong, Yan,Yu, Runxian,Chen, Jingfang,et al. Highly active repeat-mediated recombination in the mitogenome of the holoparasitic plant Aeginetia indica[J]. FRONTIERS IN PLANT SCIENCE,2022,13.
APA	Zhong, Yan,Yu, Runxian,Chen, Jingfang,Liu, Ying,&Zhou, Renchao.(2022).Highly active repeat-mediated recombination in the mitogenome of the holoparasitic plant Aeginetia indica.FRONTIERS IN PLANT SCIENCE,13.
MLA	Zhong, Yan,et al."Highly active repeat-mediated recombination in the mitogenome of the holoparasitic plant Aeginetia indica".FRONTIERS IN PLANT SCIENCE 13(2022).

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