Cell-free supernatant of Bacillus velezensis suppresses mycelial growth and reduces virulence of Botrytis cinerea by inducing oxidative stress

doi:10.3389/fmicb.2022.980022

	Cell-free supernatant of Bacillus velezensis suppresses mycelial growth and reduces virulence of Botrytis cinerea by inducing oxidative stress
	Zhao, Huanlan ; Liu, Kui 1; Fan, Yezhen ; Cao, Jiacan 2; Li, Huanghuan 3; Song, Wu ; Liu, Yongsheng 4,5; Miao, Min
	2022
发表期刊	FRONTIERS IN MICROBIOLOGY
卷号	13
摘要	As a notorious pathogenic fungus, Botrytis cinerea has been reported to infect more than 1400 species of plants and cause postharvest gray mold of numerous economic fruits, leading to substantial economic losses. Traditional chemical fungicides in pathogen control have potential issues regarding environmental pollution, disease resistance and human health. More safety and efficacious prevention technique of postharvest gray mold are in urgent demand. This study aims to investigate the potential function and mechanism of Bacillus velezensis to control gray mold for harvested fruits. The results showed that the cell-free supernatant (CFS) generated from B. velezensis strain A4 was able to inhibit spore germination, germ tube elongation and hyphal growth of B. cinerea in vitro, and impair the pathogenicity of B. cinerea on the four tested fruits. Further analysis demonstrated that CFS significantly reduced the expression of genes associated with growth and pathogenicity and weakened the ability of B. cinerea spores to penetrate plant cell walls in a dose-dependent manner. Moreover, the CFS destroyed the membrane of hyphae, resulting in exosmosis of cell contents and caused hyphal cells to accumulate excessive reactive oxygen species (ROS), leading to hyphal oxidative damage. Our findings indicate that B. velezensis CFS can damage B. cinerea mycelial cells by promoting excessive accumulation of ROS to realize its biological control function.
关键词	Botrytis cinerea Bacillus velezensis cell-free supernatant reactive oxygen species damage of cell membrane
学科领域	Microbiology
DOI	10.3389/fmicb.2022.980022
收录类别	SCI
语种	英语
WOS关键词	TOMATO FRUIT ; BROWN-ROT ; BIOCONTROL ; JUJUBE ; MOLD ; ACCUMULATION ; PENETRATION ; MANAGEMENT ; FUNGICIDES ; RESISTANCE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000843862900001
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
EISSN	1664-302X
资助机构	National Natural Science Foundation of China ; [31970345] ; [31972474] ; [31701059]
作者邮箱	minmiao@hfut.edu.cn
作品OA属性	Green Published, gold
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28980
专题	中科院北方资源植物重点实验室
作者单位	1.Hefei Univ Technol, Sch Food & Biol Engn, Hefei, Peoples R China 2.Chinese Acad Sciencess, Inst Bot, Beijing, Peoples R China 3.Jiangnan Univ, Sch Food Sci & Technol, Wuxi, Peoples R China 4.North Minzu Univ, Sch Biol Sci & Engn, Yinchuan, Peoples R China 5.Sichuan Univ, Coll Life Sci, State Key Lab Hydraul & Mt River Engn, Minist Educ Key Lab Bioresource & Ecoenvironm, Chengdu, Peoples R China 6.Anhui Agr Univ, Sch Hort, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Huanlan,Liu, Kui,Fan, Yezhen,et al. Cell-free supernatant of Bacillus velezensis suppresses mycelial growth and reduces virulence of Botrytis cinerea by inducing oxidative stress[J]. FRONTIERS IN MICROBIOLOGY,2022,13.
APA	Zhao, Huanlan.,Liu, Kui.,Fan, Yezhen.,Cao, Jiacan.,Li, Huanghuan.,...&Miao, Min.(2022).Cell-free supernatant of Bacillus velezensis suppresses mycelial growth and reduces virulence of Botrytis cinerea by inducing oxidative stress.FRONTIERS IN MICROBIOLOGY,13.
MLA	Zhao, Huanlan,et al."Cell-free supernatant of Bacillus velezensis suppresses mycelial growth and reduces virulence of Botrytis cinerea by inducing oxidative stress".FRONTIERS IN MICROBIOLOGY 13(2022).

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