The calcium signaling module CaM-IQM destabilizes IAA-ARF interaction to regulate callus and lateral root formation

doi:10.1073/pnas.2202669119

	The calcium signaling module CaM-IQM destabilizes IAA-ARF interaction to regulate callus and lateral root formation
	Zhang, Shiqi 1; Yu, Ruixue 1; Yu, Dongxue 1; Chang, Pengjie 1; Guo, Shiqi 1; Yang, Xiaona 1; Liu, Xinchun 1; Xu, Chongyi ; Hu, Yuxin2
	2022
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN	0027-8424
卷号	119 期号:27
摘要	Induction of a pluripotent cell mass, called callus, from detached organs is an initial step in in vitro plant regeneration, during which phytohormone auxin-induced ectopic activation of a root developmental program has been shown to be required for subsequent de novo regeneration of shoots and roots. However, whether other signals are involved in governing callus formation, and thus plant regeneration capability, remains largely unclear. Here, we report that the Arabidopsis calcium (Ca2+) signaling module CALMODULIN IQ-MOTIF CONTAINING PROTEIN (CaM-IQM) interacts with auxin signaling to regulate callus and lateral root formation. We show that disruption of IQMs or CaMs retards auxin-induced callus and lateral root formation by dampening auxin responsiveness, and that CaM-IQM complexes physically interact with the auxin signaling repressors INDOLE-3-ACETIC ACID INDUCIBLE (IAA) proteins in a Ca2+-dependent manner We further provide evidence that the physical interaction of CaM6 with IAA19 destabilizes the repressive interaction of IAA19 with AUXIN RESPONSE FACTOR 7 (ARF7), and thus regulates auxin-induced callus formation. These findings not only define a critical role of CaM-IQM-mediated Ca2+ signaling in callus and lateral root formation, but also provide insight into the interplay of Ca2+ signaling and auxin actions during plant regeneration and development.
关键词	calcium auxin callus lateral root
学科领域	Multidisciplinary Sciences
DOI	10.1073/pnas.2202669119
收录类别	SCI
语种	英语
WOS关键词	CALMODULIN-BINDING PROTEIN ; MEDIATED TRANSFORMATION ; ARABIDOPSIS-THALIANA ; SHOOT DEVELOPMENT ; GENE-EXPRESSION ; AUXIN ; GROWTH ; REGENERATION ; ENCODES ; DOMAIN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000854979100014
出版者	NATL ACAD SCIENCES
文献子类	Article
出版地	WASHINGTON
EISSN	1091-6490
资助机构	National Natural Science Foundation of China [31830055, 32170317] ; Strategic Priority Research Program of Chinese Academy of Sciences [XDB27030102]
作者邮箱	xuchongyi@ibcas.ac.cn ; huyuxin@ibcas.ac.cn
作品OA属性	Green Published, hybrid
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28994
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Shiqi,Yu, Ruixue,Yu, Dongxue,et al. The calcium signaling module CaM-IQM destabilizes IAA-ARF interaction to regulate callus and lateral root formation[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2022,119(27).
APA	Zhang, Shiqi.,Yu, Ruixue.,Yu, Dongxue.,Chang, Pengjie.,Guo, Shiqi.,...&Hu, Yuxin.(2022).The calcium signaling module CaM-IQM destabilizes IAA-ARF interaction to regulate callus and lateral root formation.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,119(27).
MLA	Zhang, Shiqi,et al."The calcium signaling module CaM-IQM destabilizes IAA-ARF interaction to regulate callus and lateral root formation".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 119.27(2022).

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