Crystal Structures of Arabidopsis thaliana GDP-D-Mannose Pyrophosphorylase VITAMIN C DEFECTIVE 1

doi:10.3389/fpls.2022.899738

IB-CAS > 中科院光生物学重点实验室

	Crystal Structures of Arabidopsis thaliana GDP-D-Mannose Pyrophosphorylase VITAMIN C DEFECTIVE 1
	Zhang, Chi ; Zhao, Shun 1,3; Li, Yu-Shuai ; He, Chao 2; Wang, Xiao 2; Liu, Lin 2
	2022
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	13
摘要	Plant GDP-D-mannose pyrophosphorylase (GMPase) catalyzes a committed step in ascorbic acid biosynthesis pathway. Arabidopsis thaliana VTC1 is the first genetically characterized plant GMPase and has unique properties when compared with bacterial and animal homologs. Here we present the crystal structures of VTC1 in the unliganded and product-bound states at resolutions of 2.8 and 3.0 angstrom, respectively. VTC1 dimerizes in a same way like other known GMPases, but dodecamerizes in a previously unobserved arrangement. The interactions to GDP-D-mannose and inorganic pyrophosphate are revealed by the product-bound VTC1 structure. An in vitro GMPase activity assay confirms the regulatory role of the C-terminal left-handed beta-helix domain, and structural analyses suggest the models of VTC1 hetero-complex with its interacting proteins. The structural information advances our insights into the different mechanisms involved in VTC1 regulation.
关键词	ascorbic acid nucleotide sugar guanylyltransferase crystallography oligomerization
学科领域	Plant Sciences
DOI	10.3389/fpls.2022.899738
收录类别	SCI
语种	英语
WOS关键词	L-ASCORBIC-ACID ; STRUCTURE REFINEMENT ; BIOSYNTHESIS ; METABOLISM ; DEFICIENT ; MUTANT ; MODEL ; CYTOKINESIS ; AMMONIUM ; MUTATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000807112400001
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
作者邮箱	liulin@ahu.edu.cn
作品OA属性	gold, Green Published
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28997
专题	中科院光生物学重点实验室
作者单位	1.Anhui Univ, Sch Life Sci, Hefei, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China 3.Anhui Univ, Anhui Key Lab Modern Biomfg, Hefei, Peoples R China 4.Mem Sloan Kettering Canc Ctr, Struct Biol Program, New York, NY USA
推荐引用方式 GB/T 7714	Zhang, Chi,Zhao, Shun,Li, Yu-Shuai,et al. Crystal Structures of Arabidopsis thaliana GDP-D-Mannose Pyrophosphorylase VITAMIN C DEFECTIVE 1[J]. FRONTIERS IN PLANT SCIENCE,2022,13.
APA	Zhang, Chi,Zhao, Shun,Li, Yu-Shuai,He, Chao,Wang, Xiao,&Liu, Lin.(2022).Crystal Structures of Arabidopsis thaliana GDP-D-Mannose Pyrophosphorylase VITAMIN C DEFECTIVE 1.FRONTIERS IN PLANT SCIENCE,13.
MLA	Zhang, Chi,et al."Crystal Structures of Arabidopsis thaliana GDP-D-Mannose Pyrophosphorylase VITAMIN C DEFECTIVE 1".FRONTIERS IN PLANT SCIENCE 13(2022).

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