A MADS-box transcription factor, SlMADS1, interacts with SlMACROCALYX to regulate tomato sepal growth

doi:10.1016/j.plantsci.2022.111366

	A MADS-box transcription factor, SlMADS1, interacts with SlMACROCALYX to regulate tomato sepal growth
	Xing, Mengyang 1; Li, Hongli ; Liu, Gangshuai ; Zhu, Benzhong ; Zhu, Hongliang ; Grierson, Donald 2,3; Luo, Yunbo ; Fu, Daqi
	2022
发表期刊	PLANT SCIENCE
ISSN	0168-9452
卷号	322
摘要	In flowering plants, sepals play important roles in the development of flowers and fruit, and both processes are regulated by MADS-box (MADS) transcription factors (TFs). SlMADS1 was previously reported to act as a negative regulator of fruit ripening. In this study, expression analysis shown that its transcripts were very highly expressed during the development of sepals. To test the role of SlMADS1, we generated KO-SlMADS1 (knock-out) tomato mutants by CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9) technology and over-expression of SlMADS1 (OE-SlMADS1). The sepals and individual cells of KO-SlMADS1 mutants were significantly elongated, compared with the wild type (WT), whereas the sepals of OE-SlMADS1 tomatoes were significantly shorter and their cells were wider. RNA-seq (RNA-sequencing) of sepal samples showed that ethylene-, gibberellin-, auxin-, cytokinin-and cell wall metabolism-related genes were significantly affected in both KO-SlMADS1 and OE-SlMADS1 plants with altered sepal size. Since SlMA-CROCALYX (MC) is known to regulate the development of tomato sepals, we also studied the relationship be-tween SlMC and SlMADS1 and the result showed that SlMADS1 interacts directly with SlMC. In addition, we also found that manipulating SlMADS1 expression alters the development of tomato plant leaves, roots and plant height. These results enrich our understanding of sepal development and the function of SlMADS1 throughout the plant.
关键词	Plant hormones Sepal growth Tomato SlMADS1 SlMC Transcription factors
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1016/j.plantsci.2022.111366
收录类别	SCI
语种	英语
WOS关键词	ORGAN IDENTITY ; ETHYLENE ; AUXIN ; GENE ; XYLOGLUCAN ; EXPRESSION ; FLOWER ; BIOSYNTHESIS ; MACROCALYX ; COMPLEXES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000830264800007
出版者	ELSEVIER IRELAND LTD
文献子类	Article
出版地	CLARE
EISSN	1873-2259
资助机构	National Natural Science Foundation of China [NSFC 31571898, 32072277]
作者邮箱	daqifu@cau.edu.cn
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/29027
专题	中科院北方资源植物重点实验室
作者单位	1.China Agr Univ, Coll Food Sci & Nutr Engn, Lab Fruit Biol, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Inst Bot, Innovat Acad Seed Design, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Zhejiang Univ, Lab Fruit Qual Biol, Zhejiang Prov Key Lab Hort Plant Integrat Biol, Zijingang Campus, Hangzhou 310058, Peoples R China 4.Univ Nottingham, Sch Biosci, Plant Sci Div, Sutton Bonington Campus, Loughborough LE12 5RD, England
推荐引用方式 GB/T 7714	Xing, Mengyang,Li, Hongli,Liu, Gangshuai,et al. A MADS-box transcription factor, SlMADS1, interacts with SlMACROCALYX to regulate tomato sepal growth[J]. PLANT SCIENCE,2022,322.
APA	Xing, Mengyang.,Li, Hongli.,Liu, Gangshuai.,Zhu, Benzhong.,Zhu, Hongliang.,...&Fu, Daqi.(2022).A MADS-box transcription factor, SlMADS1, interacts with SlMACROCALYX to regulate tomato sepal growth.PLANT SCIENCE,322.
MLA	Xing, Mengyang,et al."A MADS-box transcription factor, SlMADS1, interacts with SlMACROCALYX to regulate tomato sepal growth".PLANT SCIENCE 322(2022).

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