Linolenic Acid-Derived Oxylipins Inhibit Aflatoxin Biosynthesis in Aspergillus flavus through Activation of Imizoquin Biosynthesis

doi:10.1021/acs.jafc.2c06230

	Linolenic Acid-Derived Oxylipins Inhibit Aflatoxin Biosynthesis in Aspergillus flavus through Activation of Imizoquin Biosynthesis
	Wu, Shaowen ; Zhang, Qunjie 1; Zhang, Wenyang ; Huang, Wenjie ; Kong, Qian ; Liu, Qinjian ; Li, Wenyan ; Zou, Xinlu ; Liu, Chun-Ming; Yan, Shijuan
	2022
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN	0021-8561
摘要	Oxylipins play important signaling roles in aflatoxin (AF) biosynthesis in Aspergillus flavus. We previously showed that exogenous supply of autoxidated linolenic acid (AL) inhibited AF biosynthesis in A. flavus via oxylipins, but the molecular mechanism is still unknown. Here, we performed multiomics analyses of A. flavus grown in media with or without AL. Targeted metabolite analyses and quantitative reverse transcription (qRT)-polymerase chain reaction (PCR) showed that the imizoquin (IMQ) biosynthetic pathway was distinctly upregulated in the presence of AL. C-13-glucose labeling confirmed in parallel that the tricarboxylic acid cycle was also enhanced by AL, consistent with observed increases in mycelial growth. Moreover, we integrated thermal proteome profiling and molecular dynamics simulations to identify a potential receptor of AL; AL was found to interact with a transporter (ImqJ) located in the IMQ gene cluster, primarily through hydrophobic interactions. Further analyses of strains with an IMQ pathway transcription factor overexpressed or knocked out confirmed that this pathway was critical for AL-mediated inhibition of AF biosynthesis. Comparison of 22 assembled A. flavus and Aspergillus oryzae genomes showed that genes involved in the IMQ pathway were positively selected in A. oryzae. Taken together, the results of our study provide novel insights into oxylipin-mediated regulation of AF biosynthesis and suggest potential methods for preventing AF contamination of crops.
关键词	Aspergillus flavus aflatoxins imizoquin multiomics thermal proteome profiling
学科领域	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
DOI	10.1021/acs.jafc.2c06230
收录类别	SCI
语种	英语
WOS关键词	SIGNALING PATHWAYS ; SOFTWARE NEWS ; FATTY-ACIDS ; DYNAMICS ; PROTEIN ; GENE ; IDENTIFICATION ; METABOLITES ; ANTAGONISM ; MODELS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000895983800001
出版者	AMER CHEMICAL SOC
文献子类	Article; Early Access
出版地	WASHINGTON
EISSN	1520-5118
资助机构	Natural Science Foundation of China ; Special Fund for Scientific Innovation Strategy-Construction of High Level Academy of Agriculture Science ; Natural Science Foundation of Guangdong Province ; Guangdong Special Support 318 Program ; [31500045] ; [R2021YJ-YB1004] ; [R2020PY-JX019] ; [2014A030310489] ; [2021TQ06N115]
作者邮箱	shijuan@agrogene.ac.cn
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/29030
专题	中科院植物分子生理学重点实验室
作者单位	1.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Key Lab Crop Germplasm Resources Preserv, Guangzhou 510640, Peoples R China 2.South China Agr Univ, Inst Genom & Bioinformat, Guangzhou 510642, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Wu, Shaowen,Zhang, Qunjie,Zhang, Wenyang,et al. Linolenic Acid-Derived Oxylipins Inhibit Aflatoxin Biosynthesis in Aspergillus flavus through Activation of Imizoquin Biosynthesis[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2022.
APA	Wu, Shaowen.,Zhang, Qunjie.,Zhang, Wenyang.,Huang, Wenjie.,Kong, Qian.,...&Yan, Shijuan.(2022).Linolenic Acid-Derived Oxylipins Inhibit Aflatoxin Biosynthesis in Aspergillus flavus through Activation of Imizoquin Biosynthesis.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY.
MLA	Wu, Shaowen,et al."Linolenic Acid-Derived Oxylipins Inhibit Aflatoxin Biosynthesis in Aspergillus flavus through Activation of Imizoquin Biosynthesis".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022).

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