Comparative Microbial Nitrogen Functional Gene Abundances in the Topsoil vs. Subsoil of Three Grassland Habitats in Northern China

doi:10.3389/fpls.2021.792002

	Comparative Microbial Nitrogen Functional Gene Abundances in the Topsoil vs. Subsoil of Three Grassland Habitats in Northern China
	Liu, Yuqing 1,2; Chi, Qiaodong 2; Cheng, Hui 2; Ding, Huanxin 6; Wen, Teng 2; Zhao, Jun 2,4,5; Feng, Xiaojuan3 ; Zhang, Jinbo 2,4,5; Cai, Zucong 2,5,7; Liu, Guohua
	2022
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	12
摘要	The microbial groups of nitrogen fixers, ammonia oxidizers, and denitrifiers play vital roles in driving the nitrogen cycle in grassland ecosystems. However, the understanding of the abundance and distribution of these functional microorganisms as well as their driving factors were limited mainly to topsoil. In this study, the abundances of nitrogen functional genes (NFGs) involved in nitrogen fixation (nifH), ammonia oxidation (amoA), and denitrification (nirK, nirS, and nosZ) were investigated in both topsoil (0-10 cm, soil layer with concentrated root) and subsoil (30-40 cm, soil layer with spare root) of three grassland habitats in northern China. The abundance of NFGs decreased with soil depth except for the archaeal amoA gene and the distribution of nifH, archaeal amoA, nirK, and nirS gene was significantly impacted by grassland habitats. Moreover, the distribution of NFGs was more responsive to the vertical difference than horizontal spatial heterogeneity. Redundancy analysis revealed that the distribution pattern of overall NFGs was regulated by grassland habitats, and these regulations were more obvious in the subsoil than in the topsoil. Variance partitioning analysis further indicated that soil resource supply (e.g., organic matter) may control the vertical distribution of NFGs. Taken together, the findings in this study could fundamentally improve our understanding of the distribution of N cycling-associated microorganisms across a vertical scale, which would be useful for predicting the soil N availability and guiding the soil N management in grassland ecosystems.
关键词	grassland ecosystem nitrogen functional gene soil depth real-time PCR abundance
学科领域	Plant Sciences
DOI	10.3389/fpls.2021.792002
收录类别	SCI
语种	英语
WOS关键词	COMMUNITY STRUCTURE ; DENITRIFYING BACTERIA ; TIBETAN ALPINE ; SOIL DEPTH ; TEMPERATE GRASSLANDS ; AMMONIA-OXIDIZERS ; GLOBAL PATTERNS ; DIVERSITY ; PLANT ; GRADIENT
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000752699700001
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
资助机构	National Natural Science Foundation of China [41977084, 41977032] ; Key-Area Research and Development Program of Guangdong Province [2020B0202010006] ; Opening Project of Zhejiang Key Laboratory of Urban Environmental Processes and Pollution Control [NUEORS202002]
作者邮箱	wenteng@njnu.edu.cn ; junzhao37@njnu.edu.cn
作品OA属性	Green Published, gold
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/29051
专题	植被与环境变化国家重点实验室
作者单位	1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China 3.Nanjing Normal Univ, Sch Geog, Nanjing, Peoples R China 4.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing, Peoples R China 5.Jiangsu Ctr Collaborat Innovat Geog Informat Reso, Nanjing, Peoples R China 6.Zhongke Clean Soil Technol Serv Co Ltd, Guangzhou, Peoples R China 7.Suzhou Stn Farmland Qual Protect, Suzhou, Peoples R China 8.Nanjing Normal Univ, Key Lab Virtual Geog Environm, Minist Educ, Nanjing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yuqing,Chi, Qiaodong,Cheng, Hui,et al. Comparative Microbial Nitrogen Functional Gene Abundances in the Topsoil vs. Subsoil of Three Grassland Habitats in Northern China[J]. FRONTIERS IN PLANT SCIENCE,2022,12.
APA	Liu, Yuqing.,Chi, Qiaodong.,Cheng, Hui.,Ding, Huanxin.,Wen, Teng.,...&Liu, Guohua.(2022).Comparative Microbial Nitrogen Functional Gene Abundances in the Topsoil vs. Subsoil of Three Grassland Habitats in Northern China.FRONTIERS IN PLANT SCIENCE,12.
MLA	Liu, Yuqing,et al."Comparative Microbial Nitrogen Functional Gene Abundances in the Topsoil vs. Subsoil of Three Grassland Habitats in Northern China".FRONTIERS IN PLANT SCIENCE 12(2022).

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