Exogenous Arachidonic Acid Affects Fucoxanthin Biosynthesis and Photoprotection in Phaeodactylum tricornutum

doi:10.3390/md20100644

IB-CAS > 中科院光生物学重点实验室

	Exogenous Arachidonic Acid Affects Fucoxanthin Biosynthesis and Photoprotection in Phaeodactylum tricornutum
	Zhu, Shuaiqi 1; Bin, Song 1; Wang, Wenda 1; Lu, Shan ; Yang, Wenqiang1,2,3
	2022
发表期刊	MARINE DRUGS
卷号	20 期号:10
摘要	Fucoxanthin is an oxygenated carotenoid component that has been reported to play important roles in anti-oxidation, anti-obesity and anti-cancer in the human body. Fucoxanthin-chlorophyll protein (FCP) complexes participate in light harvesting and photoprotection in diatom. In order to better understand the change of fucoxanthin content and its role in photoprotection, the growth, fucoxanthin biosynthesis and photosynthetic phenotypes were studied in the diatom Phaeodactylum tricornutum under the treatment of exogenous arachidonic acid (AA). Our results demonstrated that even low concentration of AA at 0.1 mg/L strongly induced fucoxanthin accumulation in algal cells to a maximum of 1.1 mg/g dry weight (DW), which was 36.6% higher than that in the untreated ones. By principal component analysis (PCA), we also identified a close correlation between fucoxanthin accumulation and the expression of genes involved in fucoxanthin biosynthesis, especially phytoene synthase (PSY), suggesting that AA change the metabolism of fucoxanthin by inducing carotenoid metabolic enzymes at the transcriptional level. Furthermore, we found that the exogenous application of AA affected non-photochemical quenching (NPQ) and photoinhibition, which resulted from the changed diadinoxanthin (DD) and diatoxanthin (DT) cycle, and thus played an important role in photoprotection.
关键词	gene expression carotenoid principal component analysis light harvesting non-photochemical quenching
学科领域	Chemistry, Medicinal ; Pharmacology & Pharmacy
DOI	10.3390/md20100644
收录类别	SCI
语种	英语
WOS关键词	CAROTENOID BIOSYNTHESIS ; CHLOROPHYLL PROTEIN ; PHOTOSYSTEM-I ; EXPRESSION ; PATHWAY ; IDENTIFICATION ; DIADINOXANTHIN ; INHIBITION ; METABOLISM ; REVEALS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000873065800001
出版者	MDPI
文献子类	Article
出版地	BASEL
EISSN	1660-3397
资助机构	Natural Key R&D Program of China [2021YFA0910800, 2021YFA0909600, 2019YFA0904600]
作者邮箱	shanlu@nju.edu.cn ; wqyang@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/29065
专题	中科院光生物学重点实验室
作者单位	1.Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210023, Peoples R China 2.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Shuaiqi,Bin, Song,Wang, Wenda,et al. Exogenous Arachidonic Acid Affects Fucoxanthin Biosynthesis and Photoprotection in Phaeodactylum tricornutum[J]. MARINE DRUGS,2022,20(10).
APA	Zhu, Shuaiqi,Bin, Song,Wang, Wenda,Lu, Shan,&Yang, Wenqiang.(2022).Exogenous Arachidonic Acid Affects Fucoxanthin Biosynthesis and Photoprotection in Phaeodactylum tricornutum.MARINE DRUGS,20(10).
MLA	Zhu, Shuaiqi,et al."Exogenous Arachidonic Acid Affects Fucoxanthin Biosynthesis and Photoprotection in Phaeodactylum tricornutum".MARINE DRUGS 20.10(2022).

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