Global ubiquitinome analysis reveals the role of E3 ubiquitin ligase FaBRIZ in strawberry fruit ripening

doi:10.1093/jxb/erac400

	Global ubiquitinome analysis reveals the role of E3 ubiquitin ligase FaBRIZ in strawberry fruit ripening
	Wang, Yuying 1; Kong, Lingxi 2; Wang, Weihao 1; Qin, Guozheng1,2
	2023
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	74 期号:1 页码:214-232
摘要	Using ubiquitinome analysis we unravel several hundreds of ubiquitination sites on proteins in strawberry fruit, and identify a RING-type E3 ligase, FaBRIZ, which functions as an important negative regulator of ripening. Ubiquitination is an important post-translational modification that mediates protein degradation in eukaryotic cells, participating in multiple biological processes. However, the profiling of protein ubiquitination and the function of this crucial modification in fruit ripening remain largely unknown. In this study, we found that suppression of proteasome by the inhibitor MG132 retarded strawberry fruit ripening. Using K-e-GG antibody enrichment combined with high-resolution mass spectrometry, we performed a comprehensive ubiquitinome analysis in strawberry fruit. We identified 2947 ubiquitination sites for 2878 peptides within 1487 proteins, which are involved in a variety of cellular functions. The lysine at position 48 (K48)-linked poly-ubiquitin chains appeared to be the most prevalent type of modification among the identified ubiquitinated proteins. A large number of ubiquitination sites exhibited altered ubiquitination levels after proteasome inhibition, including those within ripening-related proteins associated with sugar and acid metabolism, cell wall metabolism, anthocyanin synthesis, and ABA biosynthesis and signalling. We further demonstrated that FaBRIZ, a RING-type E3 ligase, functions as a negative regulator of ripening in strawberry fruit. Our findings highlight the critical regulatory roles of protein ubiquitination in fruit ripening. The ubiquitinome data provide a basis for further exploration of the function of ubiquitination on specific proteins.
关键词	Fruit ripening protein post-translational modification strawberry ubiquitination ubiquitinome ubiquitin-proteasome system (UPS)
学科领域	Plant Sciences
DOI	10.1093/jxb/erac400
收录类别	SCI
语种	英语
WOS关键词	TRANSCRIPTION FACTOR ; PROTEIN-DEGRADATION ; GENE-EXPRESSION ; TOMATO ; PROTEASOME ; ETHYLENE ; DEHYDROGENASE ; BIOSYNTHESIS ; INVOLVEMENT ; INHIBITOR
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000879103300001
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	National Natural Science Foundation of China [31925035, 32072636] ; National Key Research and Development Program [2018YFD1000200]
作者邮箱	wangweihao@ibcas.ac.cn ; gzqin@ibcas.ac.cn
作品OA属性	hybrid, Green Published
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/29088
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.China Natl Bot Garden, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yuying,Kong, Lingxi,Wang, Weihao,et al. Global ubiquitinome analysis reveals the role of E3 ubiquitin ligase FaBRIZ in strawberry fruit ripening[J]. JOURNAL OF EXPERIMENTAL BOTANY,2023,74(1):214-232.
APA	Wang, Yuying,Kong, Lingxi,Wang, Weihao,&Qin, Guozheng.(2023).Global ubiquitinome analysis reveals the role of E3 ubiquitin ligase FaBRIZ in strawberry fruit ripening.JOURNAL OF EXPERIMENTAL BOTANY,74(1),214-232.
MLA	Wang, Yuying,et al."Global ubiquitinome analysis reveals the role of E3 ubiquitin ligase FaBRIZ in strawberry fruit ripening".JOURNAL OF EXPERIMENTAL BOTANY 74.1(2023):214-232.

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