Male-linked gene TsRPL10a′ in androdioecious tree Tapiscia sinensis: implications for sex differentiation by influencing gynoecium development

doi:10.1093/treephys/tpac131

	Male-linked gene TsRPL10a′ in androdioecious tree Tapiscia sinensis: implications for sex differentiation by influencing gynoecium development
	Wang, Feng ; Ren, Xiaolong 1; Jiang, Minggao ; Hou, Kunpeng ; Xin, Guiliang ; Yan, Feng ; Zhao, Peng ; Liu, Wenzhe
	2023
发表期刊	TREE PHYSIOLOGY
ISSN	0829-318X
卷号	43 期号:3 页码:486-500
摘要	The mechanism of sex differentiation in androdioecy is of great significance for illuminating the origin and evolution of dioecy. Tapiscia sinensis Oliv. is a functionally androdioecious species with both male and hermaphroditic individuals. Male flowers of T. sinensis lack the ovules of gynoecia compared with hermaphrodites. To identify sex simply and accurately, and further find the potential determinants of sex differentiation in T. sinensis, we found that TsRPL10a ', a duplicate of TsRPL10a, was a male-linked gene. The promoter (5 ' untranslated region and the first intron) of TsRPL10a ' can be used to accurately identify sex in T. sinensis. TsRPL10a is a ribosomal protein that is involved in gynoecium development, and sufficient ribosomal levels are necessary for female gametogenesis. The expression level of TsRPL10a was significantly downregulated in male flower primordia compared with hermaphrodites. The RNA fluorescence in situ hybridization (FISH) assay demonstrated that TsRPL10a was almost undetectable in male gynoecia at the gynoecial ridge stage, which was a key period of ovule formation by scanning electron microscope observation. In male flowers, although the promoter activity of TsRPL10a was significantly higher than TsRPL10a ' verified by transgenic Arabidopsis thaliana, the transcriptional expression ratio of TsRPL10a was obviously lower than TsRPL10a ' and reached its lowest at the gynoecial ridge stage, indicating the existence of a female suppressor. The promoter similarity of TsRPL10a and TsRPL10a ' was only 45.29%; the genomic sequence similarity was 89.8%; four amino acids were altered in TsRPL10a '. The secondary structure of TsRPL10a ' was different from TsRPL10a, and TsRPL10a ' did not exhibit FISH and GUS expression in the gynoecium the way TsRPL10a did. From the perspective of RT-qPCR, its high expression level, followed by the low expression level of TsRPL10a in male flowers, indicates its antagonism function with TsRPL10a. The evolutionary analysis, subcellular localization and flower expression pattern suggested that TsRPL10a might be functionally conserved with AtRPL10aA, AtRPL10aB and AtRPL10aC in A. thaliana. Overall, we speculated that TsRPL10a and its duplicate TsRPL10a ' might be involved in sex differentiation by influencing gynoecium development in T. sinensis.
关键词	functional androdioecy gynoecium development male-linked gene sex differentiation Tapiscia sinensis TsRPL10a
学科领域	Forestry
DOI	10.1093/treephys/tpac131
收录类别	SCI
语种	英语
WOS关键词	FEMALE GAMETOPHYTE DEVELOPMENT ; L10 RIBOSOMAL-PROTEINS ; SEMI-DOMINANT MUTATION ; ARABIDOPSIS ; EVOLUTION ; DIOECY ; PHOSPHORYLATION ; GAMETOGENESIS ; TRANSLATION ; MAINTENANCE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000904679100001
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1758-4469
作者邮箱	nwafufengwang@163.com ; rxlxbdx@163.com ; 202121658@stumail.nwu.edu.cn ; houkunpeng1112@163.com ; xglnuw@163.com ; fengyan115@163.com ; pengzhao@nwu.edu.cn ; lwenzhe@nwu.edu.cn
引用统计
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/29107
专题	系统与进化植物学国家重点实验室
作者单位	1.Northwest Univ, Minist Educ, Sch Life Sci, Key Lab Resource Biol & Biotechnol Western China, Xian 710069, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Wang, Feng,Ren, Xiaolong,Jiang, Minggao,等. Male-linked gene TsRPL10a′ in androdioecious tree Tapiscia sinensis: implications for sex differentiation by influencing gynoecium development[J]. TREE PHYSIOLOGY,2023,43(3):486-500.
APA	Wang, Feng.,Ren, Xiaolong.,Jiang, Minggao.,Hou, Kunpeng.,Xin, Guiliang.,...&Liu, Wenzhe.(2023).Male-linked gene TsRPL10a′ in androdioecious tree Tapiscia sinensis: implications for sex differentiation by influencing gynoecium development.TREE PHYSIOLOGY,43(3),486-500.
MLA	Wang, Feng,et al."Male-linked gene TsRPL10a′ in androdioecious tree Tapiscia sinensis: implications for sex differentiation by influencing gynoecium development".TREE PHYSIOLOGY 43.3(2023):486-500.

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