MYB30 and MYB14 form a repressor-activator module with WRKY8 that controls stilbene biosynthesis in grapevine

doi:10.1093/plcell/koac308

	MYB30 and MYB14 form a repressor-activator module with WRKY8 that controls stilbene biosynthesis in grapevine
	Mu, Huayuan 1,2,3; Li, Yang 2,3; Yuan, Ling 4,5,6; Jiang, Jinzhu ; Wei, Yongzan ; Duan, Wei 2,3; Fan, Peige 2,3; Li, Shaohua2,3 ; Liang, Zhenchang 2,3; Wang, Lijun 2,3
	2023
发表期刊	PLANT CELL
ISSN	1040-4651
卷号	35 期号:1 页码:552-573
摘要	When exposed to pathogen infection or ultraviolet (UV) radiation, grapevine (Vitis vinifera) plants rapidly accumulate the stilbenoid resveratrol (Res) with concomitant increase of stilbene synthase (STS), the key enzyme in stilbene biosynthesis. Although a few transcription factors have been shown to regulate STSs, the molecular mechanism governing the regulation of STSs is not well elucidated. Our previous work showed that a VvMYB14-VvWRKY8 regulatory loop fine-tunes stilbene biosynthesis in grapevine through protein-protein interaction; overexpression of VvWRKY8 down-regulates VvMYB14 and VvSTS15/21; and application of exogenous Res up-regulates WRKY8 expression. Here, we identified an R2R3-MYB repressor, VvMYB30, which competes with the activator VvMYB14 for binding to the common binding sites in the VvSTS15/21 promoter. Similar to VvMYB14, VvMYB30 physically interacts with VvWRKY8 through their N-termini, forming a complex that does not bind DNA. Exposure to UV-B/C stress induces VvMYB14, VvWRKY8, and VvSTS15/21, but represses VvMYB30 in grapevine leaves. In addition, MYB30 expression is up-regulated by VvWRKY8-overexpression or exogenous Res. These findings suggest that the VvMYB14-VvWRKY8-VvMYB30 regulatory circuit allows grapevine to respond to UV stress by producing Res and prevents over-accumulation of Res to balance metabolic costs. Our work highlights the stress-mediated induction and feedback inhibition of stilbene biosynthesis through a complex regulatory network involving multiple positive and negative transcriptional regulators. VvMYB30 is involved in a complex regulatory network containing multiple positive and negative transcriptional regulators that fine-tunes stilbene biosynthesis under ultraviolet stress.
学科领域	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
DOI	10.1093/plcell/koac308
收录类别	SCI
语种	英语
WOS关键词	UV-C TREATMENTS ; TRANSCRIPTION FACTORS ; ANTHOCYANIN BIOSYNTHESIS ; VITIS-VINIFERA ; NEGATIVE REGULATOR ; R2R3-MYB GENE ; RESVERATROL ; EXPRESSION ; SYNTHASE ; BERRIES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000876347800001
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	1532-298X
资助机构	National Key R&D Program of China [2019YFD1000101] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA23080602] ; National Natural Science Foundation of China [31672120]
作者邮箱	ljwang@ibcas.ac.cn
作品OA属性	Green Published
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/29112
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Natl Bot Garden, Beijing 100093, Peoples R China 4.LIA INNOGRAPE Int Associated Lab, Beijing 100093, Peoples R China 5.Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA 6.Chinese Acad Sci, Key Lab South China Agr Plant Mol Anal & Genet Im, South China Bot Garden, Guangzhou 510650, Peoples R China 7.Chinese Acad Sci, Guangdong Prov Key Lab Appl Bot, South China Bot Garden, Guangzhou 510650, Peoples R China
推荐引用方式 GB/T 7714	Mu, Huayuan,Li, Yang,Yuan, Ling,et al. MYB30 and MYB14 form a repressor-activator module with WRKY8 that controls stilbene biosynthesis in grapevine[J]. PLANT CELL,2023,35(1):552-573.
APA	Mu, Huayuan.,Li, Yang.,Yuan, Ling.,Jiang, Jinzhu.,Wei, Yongzan.,...&Wang, Lijun.(2023).MYB30 and MYB14 form a repressor-activator module with WRKY8 that controls stilbene biosynthesis in grapevine.PLANT CELL,35(1),552-573.
MLA	Mu, Huayuan,et al."MYB30 and MYB14 form a repressor-activator module with WRKY8 that controls stilbene biosynthesis in grapevine".PLANT CELL 35.1(2023):552-573.

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