Arabidopsis EXECUTER1 interacts with WRKY transcription factors to mediate plastid-to-nucleus singlet oxygen signaling

doi:10.1093/plcell/koac330

IB-CAS > 中科院光生物学重点实验室

	Arabidopsis EXECUTER1 interacts with WRKY transcription factors to mediate plastid-to-nucleus singlet oxygen signaling
	Li, Yuhong ; Liu, Hanhong ; Ma, Tingting ; Li, Jialong ; Yuan, Jiarui ; Xu, Yong-Chao ; Sun, Ran ; Zhang, Xinyu 3; Jing, Yanjun ; Guo, Ya-Long; Lin, Rongcheng1
	2023
发表期刊	PLANT CELL
ISSN	1040-4651
卷号	35 期号:2 页码:827-851
摘要	Chloroplasts produce singlet oxygen (O-1(2)), which causes changes in nuclear gene expression through plastid-to-nucleus retrograde signaling to increase plant fitness. However, the identity of this O-1(2)-triggered pathway remains unclear. Here, we identify mutations in GENOMES UNCOUPLED4 (GUN4) and GUN5 as suppressors of phytochrome-interacting factor1 (pif1) pif3 in regulating the photo-oxidative response in Arabidopsis thaliana. GUN4 and GUN5 specifically interact with EXECUTER1 (EX1) and EX2 in plastids, and this interaction is alleviated by treatment with Rose Bengal (RB) or white light. Impaired expression of GUN4, GUN5, EX1, or EX2 leads to insensitivity to excess light and overexpression of EX1 triggers photo-oxidative responses. Strikingly, upon light irradiation or RB treatment, EX1 transiently accumulates in the nucleus and the nuclear fraction of EX1 shows a similar molecular weight as the plastid-located protein. Point mutagenesis analysis indicated that nuclear localization of EX1 is required for its function. EX1 acts as a transcriptional co-activator and interacts with the transcription factors WRKY18 and WRKY40 to promote the expression of O-1(2)-responsive genes. This study suggests that EX1 may act in plastid-to-nucleus signaling and establishes a O-1(2)-triggered retrograde signaling pathway that allows plants adapt to changing light environments during chloroplast development. EX1 acts as a plastid-to-nucleus mobile protein that relays O-1(2) signals, thus bridging the communication gap between plastids and the nucleus.
学科领域	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
DOI	10.1093/plcell/koac330
收录类别	SCI
语种	英语
WOS关键词	CHLOROPHYLL BIOSYNTHESIS ; GENE-EXPRESSION ; STRESS RESPONSES ; PROTEIN INTERACTIONS ; MG-CHELATASE ; DNA-BINDING ; CHLOROPLAST ; LIGHT ; REGULATOR ; REVEALS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000927607300001
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	1532-298X
作者邮箱	rclin@ibcas.ac.cn
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/29120
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 4.ENS Lyon, Lab Reprod & Dev Plantes, Lyon, France
推荐引用方式 GB/T 7714	Li, Yuhong,Liu, Hanhong,Ma, Tingting,et al. Arabidopsis EXECUTER1 interacts with WRKY transcription factors to mediate plastid-to-nucleus singlet oxygen signaling[J]. PLANT CELL,2023,35(2):827-851.
APA	Li, Yuhong.,Liu, Hanhong.,Ma, Tingting.,Li, Jialong.,Yuan, Jiarui.,...&Lin, Rongcheng.(2023).Arabidopsis EXECUTER1 interacts with WRKY transcription factors to mediate plastid-to-nucleus singlet oxygen signaling.PLANT CELL,35(2),827-851.
MLA	Li, Yuhong,et al."Arabidopsis EXECUTER1 interacts with WRKY transcription factors to mediate plastid-to-nucleus singlet oxygen signaling".PLANT CELL 35.2(2023):827-851.

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