Cytosolic disproportionating enzyme2 is essential for pollen germination and pollen tube elongation in rice

doi:10.1093/plphys/kiac496

	Cytosolic disproportionating enzyme2 is essential for pollen germination and pollen tube elongation in rice
	Chen, Liangke 1; Dong, Xiangbai ; Yang, Huifang 1; Chai, Yaru 1; Xia, Yan 1; Tian, Lihong ; Qu, Le Qing 1
	2023
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	191 期号:1 页码:96-109
摘要	Cytosolic DISPROPORTIONATING ENZYME2 converts maltose to glucose, providing energy and cellular materials for pollen germination and pollen tube elongation in rice. Degradation of starch accumulated in pollen provides energy and cellular materials for pollen germination and pollen tube elongation. Little is known about the function of cytosolic disproportionating enzyme2 (DPE2) in rice (Oryza sativa). Here, we obtained several DPE2 knockout mutant (dpe2) lines via genomic editing and found that the mutants grew and developed normally but with greatly reduced seed-setting rates. Reciprocal crosses between dpe2 and wild-type plants demonstrated that the mutant was male sterile. In vitro and in vivo examinations revealed that the pollen of the dpe2 mutant developed and matured normally but was defective in germination and elongation. DPE2 deficiency increased maltose content in pollen, whereas it reduced the levels of starch, glucose, fructose, and adenosine triphosphate (ATP). Exogenous supply of glucose or ATP to the germination medium partially rescued the pollen germination defects of dpe2. The expression of cytosolic phosphorylase2 (Pho2) increased significantly in dpe2 pollen. Knockout of Pho2 resulted in a semi-sterile phenotype. We failed to obtain homozygous dpe2 pho2 double mutant lines. Our results demonstrate that maltose catalyzed by DPE2 to glucose is the main energy source for pollen germination and pollen tube elongation, while Pho2 might partially compensate for deficiency of DPE2.
学科领域	Plant Sciences
DOI	10.1093/plphys/kiac496
收录类别	SCI
语种	英语
WOS关键词	MALTOSE METABOLISM ; STARCH SYNTHESIS ; GENE-EXPRESSION ; SUCROSE ; LEAVES ; DEGRADATION ; MATURATION ; REPRESSION ; ANTHER ; LEADS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000879842400001
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	1532-2548
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences [XDA24010401] ; Natural Science Foundation of China [31771697]
作者邮箱	lqqu@ibcas.ac.cn
作品OA属性	Green Published
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/29125
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Chen, Liangke,Dong, Xiangbai,Yang, Huifang,et al. Cytosolic disproportionating enzyme2 is essential for pollen germination and pollen tube elongation in rice[J]. PLANT PHYSIOLOGY,2023,191(1):96-109.
APA	Chen, Liangke.,Dong, Xiangbai.,Yang, Huifang.,Chai, Yaru.,Xia, Yan.,...&Qu, Le Qing.(2023).Cytosolic disproportionating enzyme2 is essential for pollen germination and pollen tube elongation in rice.PLANT PHYSIOLOGY,191(1),96-109.
MLA	Chen, Liangke,et al."Cytosolic disproportionating enzyme2 is essential for pollen germination and pollen tube elongation in rice".PLANT PHYSIOLOGY 191.1(2023):96-109.

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