Extensive regulation of pH-responsive transcription factor PacC on secondary metabolism contributes to development and virulence of Botrytis cinerea

doi:10.1016/j.postharvbio.2022.112219

	Extensive regulation of pH-responsive transcription factor PacC on secondary metabolism contributes to development and virulence of Botrytis cinerea
	Zhuo, Ruiling 1,2; Li, Guangjin 1,2; Peng, Huaimin ; Zong, Yuanyuan ; Wang, Xiao ; Lu, Shasha 1,2; Chen, Yong 1; Zhang, Zhanquan 1; Tian, Shiping1,2 ; Li, Boqiang 1
	2023
发表期刊	POSTHARVEST BIOLOGY AND TECHNOLOGY
ISSN	0925-5214
卷号	197
摘要	Botrytis cinerea is one of the most important postharvest pathogens and causes huge economic losses worldwide. Ambient pH is a critical factor influencing virulence of B. cinerea. In this study, BcpacC, a crucial gene encoding a pH-responsive transcription factor, was knocked out in B. cinerea strain B05.10. Deletion of BcpacC resulted in pH-dependent reduction in mycelial growth, sporulation, and virulence in apple and tomato fruit, and romaine lettuce leaves. An iTRAQ based proteomic analysis identified 70 differential proteins involved in various biological processes between wildtype (WT) and Delta BcpacC mutant under pH 6 condition, including 18 proteins related to secondary metabolism. Gene expression analysis indicates that 37 of all the 42 secondary metabolism key enzyme encoding genes were regulated by BcPacC. Further, three PacC-regulated polyketide synthase encoding genes, Bcpks4, Bcpks5 and Bcpks11, were knocked out and first proved to contribute to sporulation and virulence in B. cinerea. Our results revealed that the extensive regulation of BcPacC on secondary metabolism might play crucial roles in development and pathogenicity of B. cinerea during adaption to ambient pH.
关键词	Ambient pH Botrytis cinerea PacC Polyketide synthases Secondary metabolism
学科领域	Agronomy ; Food Science & Technology ; Horticulture
DOI	10.1016/j.postharvbio.2022.112219
收录类别	SCI
语种	英语
WOS关键词	AMBIENT PH ; POLYKETIDE SYNTHASE ; GENE-EXPRESSION ; BIOSYNTHESIS ; PATHOGENICITY ; SECRETION ; GROWTH ; FUNGI ; ENVIRONMENT ; PATHOTYPE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000904084400002
出版者	ELSEVIER
文献子类	Article
出版地	AMSTERDAM
EISSN	1873-2356
资助机构	National Natural Science Foundation of China [32072273, 31722043, 31930086] ; National Key Research and Development Program of China [2021YFD2100501] ; Youth Innovation Promotion Association, CAS [Y201919]
作者邮箱	bqli@ibcas.ac.cn
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/29140
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.China Natl Bot Garden, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhuo, Ruiling,Li, Guangjin,Peng, Huaimin,et al. Extensive regulation of pH-responsive transcription factor PacC on secondary metabolism contributes to development and virulence of Botrytis cinerea[J]. POSTHARVEST BIOLOGY AND TECHNOLOGY,2023,197.
APA	Zhuo, Ruiling.,Li, Guangjin.,Peng, Huaimin.,Zong, Yuanyuan.,Wang, Xiao.,...&Li, Boqiang.(2023).Extensive regulation of pH-responsive transcription factor PacC on secondary metabolism contributes to development and virulence of Botrytis cinerea.POSTHARVEST BIOLOGY AND TECHNOLOGY,197.
MLA	Zhuo, Ruiling,et al."Extensive regulation of pH-responsive transcription factor PacC on secondary metabolism contributes to development and virulence of Botrytis cinerea".POSTHARVEST BIOLOGY AND TECHNOLOGY 197(2023).

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